The development of modern mass spectrometry techniques for the analysis of beneficial and toxic bioactive compounds in food

Author(s)

Diogo Noin de Oliveira

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas
• Defense date: 2017-02-24

Examining board members

Rodrigo Ramos Catharino; Gisele Leticia Alves; Severino Matias de Alencar; Estela de Oliveira Lima; Anderson de Souza Sant'Ana

• Advisor: Rodrigo Ramos Catharino

Abstract

The urge for faster and highly efficient analytical methods is the drive force in food research and analysis. Metabolomics is a versatile platform that integrates both statistical and experimental data, providing highly reliable analytical results. Along with the modern mass spectrometric techniques, there is a very prominent scenario for the development of new and improved approaches. This project aimed at developing direct analysis methods for samples of various functional food products, such as olive oil, peanuts and chocolate through both conventional mass spectrometry (MS) and mass spectrometry imaging (MSI), with little sample preparation. Chemical characterization, degradation and adulteration processes were abundantly monitored within this scope. Ionization sources, such as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) were largely explored tools in this context. Structural elucidations were performed with the assistance of high-resolution mass spectrometry (ESI-Orbitrap). Chemical markers were either previously elected for a targeted analysis, or identified after the analytical process, as in metabolic fingerprinting (AU)

• FAPESP's process: 14/00084-2 - Identification of adulterations and simultaneous determination of nutrients and contaminants in food using direct analysis mass spectrometry and imaging
• Grantee: Diogo Noin de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate