Effecf of incorporation of Biosilicate and nanohydroxiapatite on physicomechanical properties of experimental resinous infiltrant

Author(s)

Ana Ferreira Souza

• Document type: Master's Dissertation
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2021-07-23

Examining board members

Giselle Maria Marchi; Vanessa Cavalli; Leily Macedo Firoozmand; Flavio Henrique Baggio Aguiar

• Advisor: Giselle Maria Marchi

Abstract

This work evaluated the influence of the incorporation of bioactive particles on physical-mechanical properties of experimental resinous infiltrants (ERI; 75% TEGDMA, 25% BisEMA, 1% camphorquinone and 0.5% EDAB). The groups were ERI + Biosilicate® 5 or 10% (Bio5 and Bio10), ERI + 10% nanohydroxyapatite (Hap10), ERI Pure (EP) and Icon® commercial infiltrant (DMG, Germany). The properties of degree of conversion (DC; n=7), sorption and solubility (SO; SOL; n=8), flexural strength, elasticity modulus (FS; EM; n=15), contact angle (CA; n=8), bioactivity (n=2) and penetration (n=4) were evaluated from samples of each material. For bioactivity analysis, samples of each group were characterized after storage simulated body fluid (0h and 30 days) by Scanning Electron Microscopy (SEM) and composition analysis by Energy Dispersive Spectroscopy (EDS). For penetration analysis, white spot lesions were induced in human dental enamel samples from a demineralizing solution at pH 4.3. The lesions were treated with the infiltrants of each group and the technique of indirect dye marking was performed using a Confocal Laser Scanning Microscope (CLSM). Data were normally distributed and were submitted to the one-way ANOVA test followed by Tukey, with ? < 0.01 (GraphPad Software, San Diego, USA). Icon® had lower SO and higher FS and EM than all groups. EP featured SOL and DC similar to Icon®, as well as a larger DC. Hap10 had DC and CA superior to all others. Bio5 and Bio10 showed CA similar to Icon®, superior SO and SOL and a reduction in the other properties. It was possible to observe the formation of precipitates in the groups with bioactive particles after storage for 30 days through SEM analysis. The EDS analysis showed an increase in Ca and P rates after storage. All infiltrants were able to penetrate the enamel lesions. The incorporation of Biosilicate® at 5 or 10% or 10% nanohydroxyapatite in ERI induces mineral deposition on the surface and does not compromise infiltrants penetration in early caries lesions, however, it reduces DC, FS and EM and increases SO and SOL, compromising its physico-mechanical properties (AU)

• FAPESP's process: 19/11850-1 - Effect of the incorporation of biosilicate and hydroxyapatite on experimental resin infiltrant
• Grantee: Ana Ferreira Souza
• Support Opportunities: Scholarships in Brazil - Master