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| Author(s): |
Elen Fernanda Nodari Dragoni
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Rio Claro. 2018-10-31. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro |
| Defense date: | 2018-09-03 |
| Advisor: | Maria Izabel Souza Camargo |
| Abstract | |
Ticks are currently been considered an important object of studies, once they are ectoparasites that severely spoliate their hosts and are vectors of a considerable number of pathogens. In this sense, it has become a priority to control these Arthropoda, mainly because such control has been performed with the use of synthetic chemicals, causing the emergence of resistant populations. Specific attention has been given to Rhipicephalus sanguineus sensu lato ticks, which mainly parasitize domestic dogs and are widely distributed. The search for efficient and sustainable alternatives to control these ectoparasites is ongoing. One alternative would be the application of vaccines with antigens capable of sensibilizing the host’s immune system and presenting cross-reactivity with other tick species. The objective of the present study was to evaluate the alterations in the salivary glands and midgut of Rhipicephalus sanguineus s. l. females fed for different periods against hosts immunized with salivary gland extracts from ticks of the same species (and with calreticulin), once these organs are vital for the biological success of the ectoparasite. The results showed that SGE2, SGE4 and CRT sensibilized the immune system of the hosts by producing antibodies against the extract proteins and CRT. Alterations in the morphophysiology of the salivary glands and in the midgut revealed that the immunization interfered in the synthesis and production of acid phosphatase and calreticulin, increasing the expression of death-related genes, leading the glands to suffer early degeneration. Such alterations in the analyzed organs suggested that the proteins present in the salivary glands including the CRT are candidates for the development of vaccines to control ticks. (AU) | |
| FAPESP's process: | 14/02843-8 - Hematophagy in female Rhipicephalus sanguineus L. S. (Acari: Ixodidae) and its relations: biochemical (bioactive gifts in extracts) and morphophysiological (salivary gland and gut) |
| Grantee: | Elen Fernanda Nodari |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |