Preparação, modulação, encapsulação e liberação controlada de plasma rico em plaquetas para aplicação em terapia regenerativa

Author(s)

Amanda Gomes Marcelino Perez

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química
• Defense date: 2013-08-19

Examining board members

Maria Helena Andrade Santana; Maria Vitória Lopes Brada Bentley; Sônia Maria Malmonge; Angela Cristina Malheiros Luzo; Rubens Maciel Filho

• Advisor: Maria Helena Andrade Santana; William Dias Belangero

Abstract

Platelet Rich Plasma (PRP) is an autologous, no immunogenic therapy, which is able to stimulate and accelerate tissue regeneration. The PRP quality is essential for success in clinical applications. Despite the widely use of PRP therapy in different areas of medicine, the preparation conditions described in literature are dispersed and, together with the autologous nature of PRP and the absence of characterization, turn difficult the interpretation of literature data and comparison of clinical results. Therefore, professional and researchers claim for the standardization of PRP preparation as the basis for clinical studies. However, the standardization of PRP preparations requires basic science studies, which are still scarce. As described in literature, due to the autologous nature of PRP, the challenge is not to study PRP, but the development of these studies. Aiming to overcoming these challenges, we developed a strategy to analyze, the integration of the steps in PRP preparation, to turn feasible the design of the formulations properties a function of the preparation conditions, with a reduced number of experiments. This strategy was applied to P-PRP, a type of PRP rich in platelets and poor in leukocytes. The analysis of the results allowed selecting three architectures of fibrin gels, derived from the activation of intact platelets, as representative of a wide range of conditions for platelet activation. These gels were characterized with respect to rheological properties, GFs release, clotting time and the ability to sustain the growth of human adipose-derived mesenchymal stem cell (hADSCs). In a second phase, the studies contemplated the incorporation of PRP in hyaluronic acid gels aiming protection, sustained release of growth factors and the ability to sustain the growth of hADSCs .The obtained results contribute to standardization of the P-PRP, as well as open new opportunities to the use the natural fibrin scaffolds in combination with hyaluronic acid. (AU)

• FAPESP's process: 10/17434-5 - Preparation, modulation, encapsulation and controlled release of platelet rich plasma for application in regenerative therapy
• Grantee: Amanda Gomes Marcelino Perez
• Support Opportunities: Scholarships in Brazil - Doctorate