Effect of nanostructured silver vanadate incorporation on antimicrobial activity, mechanical properties and morphology in dental ceramics

Author(s)

Carla Larissa Vidal

• Document type: Master's Dissertation
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC)
• Defense date: 2019-12-10

Examining board members

Andréa Candido dos Reis; Renata Cristina Silveira Rodrigues Ferracioli; Aline Evangelista de Souza Gabriel; Ligia Antunes Pereira Pinelli

• Advisor: Andréa Candido dos Reis

Abstract

Antimicrobial ceramics are desirable in many areas, but creating antimicrobial compounds that withstand high processing temperatures presents a challenge. Nanostructured silver vanadate decorated with silver nanoparticles (&beta;-AgVO3) emerges as an option that overcomes the limitations of nanomaterial particle dispersion. This study was aimed at evaluating the antimicrobial activity of ceramics incorporated into &beta;-AgVO3 against Streptococcus mutans, Streptococcus sobrinus, Aggregatibacter actinomycetemcomitans and Pseudomonas aeruginosa and examining related mechanical properties and incorporation patterns. The antimicrobial properties of ceramics incorporated with different percentages of &beta;-AgVO3 were investigated using XTT reduction and colony forming units (CFU) methods, and mechanical behavior was evaluated using tests for microhardness, surface roughness and fracture toughness. The &beta;-AgVO3 incorporation patterns were analyzed using scanning electron microscopy (SEM) and elemental dispersive energy analysis (EDS). Data distribution were analyzed using Shapiro-Wilk test and data were analyzed by ANOVA with Bonferroni adjustment and Kruskal-Wallis tests, followed by Dunn post-hoc and Mann-Wittney tests. Cell viability was reduced against S. mutans and S. sobrinus for IPS Inline with 5% &beta;-AgVO3 (p <0.05) and against A. actinomycetemcomitans with 2.5% of &beta;-AgVO3. CFU/mL was reduced against S. mutans and S. sobrinus for Inline IPS with 5% &beta;-AgVO3 (p<0.05). The control group\'s roughness was lower compared to the modified groups\' roughness (p<0.05). The fracture toughness of IPS Inline with 5% &beta;-AgVO3 presented the highest value (p<0.05). The Vickers microhardness of the control group was higher compared to that of the modified groups (p<0.05). SEM/EDS analysis showed that nanomaterial incorporation interferes in the material\'s morphology, but the presence of &beta;-AgVO3 in the respective addition correlations does not alter the ceramic\'s components. Thus, &beta;-AgVO3 promotes antimicrobial activity against S. mutans, S. sobrinus and A. actinomycetemcomitans and alters the ceramic\'s mechanical properties, but it does not alter the ceramic\'s inherent components. (AU)

• FAPESP's process: 18/03447-0 - Effect of the incorporation of nanostructured silver vanadate in antimicrobial activity, mechanical properties and morphology of dental ceramics
• Grantee: Carla Larissa Vidal
• Support Opportunities: Scholarships in Brazil - Master