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| Author(s): |
Elisvânia Freitas dos Santos
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas |
| Defense date: | 2011-11-17 |
| Examining board members: |
Nelson Adami Andreollo;
Valdemiro Carlos Sgarbieri;
Luiz Roberto Lopes;
Vania Aparecida Leandro Merhi;
José Luiz Braga de Aquino
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| Advisor: | Nelson Adami Andreollo |
| Abstract | |
The jejunoileal bypass is considered one of the disabsorptive surgical procedures in the treatment of morbid obesity, and lead to deficiency in the absorption of some minerals like iron, causing anemia, liver and providing greater amount of bile acids and other nutrients to the intestine not properly metabolized, which can be considered as cofactors in carcinogenesis. On the other hand, some components or functional food ingredients called prebiotics can positively influence the absorption of minerals and suppressing the production of carcinogenic metabolites, and the polydextrose it is one of these compounds. The objective of this research was to evaluate the effect of iron supplementation and / or polydextrose in iron metabolism and morphology of the intestine in rats submitted to jejunoileal bypass. Were employed 59 males rats divided into 3 groups: control-Sham group (C1), control group (C2). The animals of group C1 who underwent laparotomy with exposure of the bowel and Group (G) also underwent laparotomy and the small bowel was transected 5 cm from the duodenal-jejunal junction and a latero-lateral jejunoileal anastomosis was performed in the ileum 2 cm from the ileocecal valve. After 15 days the animals were divided into: C1-sham-control group, consisting of 6 animals received the AIN-93M diet formulated with iron; C2 - control group, consisting of 6 animals (commercial diet). G: Group operated which was subdivided into four subgroups: G3 - Group composed of 12 operated animals that received the AIN-93M diet formulated with iron; G4 - operated group, consisting of 12 animals that received the AIN-93M diet formulated with iron and with 7.5% polydextrose; G5 - operated group, consisting of 11 animals that received the AIN- 93M diet formulated with polydextrose 7.5% G6 - operated group composed of 12 animals received AIN-93M diet formulated without iron and without polydextrose. After 90 days of observation, the animals were sacrificed. We collected blood, liver and intestine for analysis and histological studies. For histopathological analysis the intestine was divided into five segments (S1, S2, S3, S4 and S5) corresponding to the terminal ileum, ascending colon, transverse, descending and rectum. Operated animals showed lower weight gain and lower feed intake. The serum levels of serum iron, ferritin, total iron- binding capacity, unsaturated iron binding capacity, transferrin saturation index and iron apparent absorption analysis showed no statistically significant difference between groups. The analysis of hemoglobin showed a statistically significant difference between groups in the final interval of 90 days and the hematocrit in intervals of 45 days and 90 days. Histopathology of the liver showed normal livers and various degrees of hepatic steatosis. Considering the presence or absence of steatosis, a statistically significant difference was observed between the groups. In the histopathological analysis of the segments of the intestine was not observed any change. Therefore, the results of this research showed that the supplementation of 7.5% polydextrose had no effect on iron absorption, serum iron and hematological levels after 90 days of jejunoileal bypass. The histological changes found in the liver were characterized as steatosis and were more pronounced in animals subjected to jejunoileal bypass. There were no histological changes in the intestinal mucosa of the segments during the study period (AU) | |