Study of enteric glial cells immunoreactive for P2X2 and P2X7 receptors in the ileum of rats subjected to ischemia and reperfusion.

Author(s)

Cristina Eusébio Mendes

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-06-24

Examining board members

Patricia Castelucci; Laura Beatriz Mesiano Maifrino; Romeu Rodrigues de Souza

• Advisor: Patricia Castelucci

Abstract

The nervous system response to various injuries involves the activation of enteric glial cells. The aim of the work was to analyze the effect of ischemia and reperfusion (I/R-i) on enteric glial cells, neurons and receptors P2X2 and P2X7. We analyzed the ileum of Control, Sham and I/R-i with 0 hour, 24 hours and 14 days of reperfusion. Double staining were performed P2X2 and P2X7 receptors with Hu and S100, density, area profile and marking of cellular proliferation. The results show double staining of neurons and enteric glial cell with the P2X2 and P2X7; density increased by glial cells and decrease of neurons immunoreactive to Hu. The area profile of enteric glial cell S100-IR showed decreased in Groups I/R-I and enteric glial cell proliferation was observed in groups I/R-i 0 hours and 24 hours. It is concluded that ischemia has led to changes in differential P2X2 and P2X7 receptors, neurons and enteric glial cells, which can cause gastrointestinal dysfunction. (AU)

• FAPESP's process: 10/12918-4 - Study of enteric glial cells immunoreactive for P2X2 and P2X7 receptors in the enteric nervous system of the ileum of rats subjected to ischemia and reperfusion
• Grantee: Cristina Eusébio Mendes
• Support type: Scholarships in Brazil - Master