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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis

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Chaim, Felipe Mendona [1] ; Sato, Daniela Tiemi [2] ; Rodrigues, Murilo Rocha [2] ; Dias, Alice Moreira [2] ; Silveira Junior, Paulo Pedroso [2] ; Pereira, Jose Aires [3] ; Real Martinez, Carlos Augusto [4, 3]
Total Authors: 7
[1] Univ Sao Francisco, Postgraduate Program Hlth Sci, BR-12900000 Braganca Paulista, SP - Brazil
[2] Univ Sao Francisco, Sch Med, BR-12900000 Braganca Paulista, SP - Brazil
[3] Univ Sao Francisco, Postgrad Program Hlth Sci, BR-12900000 Braganca Paulista, SP - Brazil
[4] Univ Estadual Campinas, Dept Surg, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Acta Cirurgica Brasileira; v. 29, n. 9, p. 544-552, SEP 2014.
Web of Science Citations: 6

PURPOSE: To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis. METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05). RESULTS: Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention. CONCLUSIONS: Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream. (AU)

FAPESP's process: 10/12492-7 - Evaluation of antioxidant effects of 5-aminosalycylic acid, N-acetylcysteine, sucralfate, curcumin and aqueous extract of Ilex paraguariensis in contente and pattern of tissue expression of intercellular junctions proteins: study in rats
Grantee:Carlos Augusto Real Martinez
Support type: Regular Research Grants