Distribution of the P2X2 receptor and chemical coding in ileal enteric neurons of obese male mice (ob/ob)

Author(s)

Mizuno, Marcia Sanae ^[1] ; Crisma, Amanda Rabello ^[2] ; Borelli, Primavera ^[2] ; Schaefer, Barbara Tavares ^[3] ; Silveira, Mariana Povoa ^[3] ; Castelucci, Patricia ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: WORLD JOURNAL OF GASTROENTEROLOGY; v. 20, n. 38, p. 13911-13919, OCT 14 2014.
• Web of Science Citations: 6

Abstract

AIM: To investigate the colocalization, density and profile of neuronal areas of enteric neurons in the ileum of male obese mice. METHODS: The small intestinal samples of male mice in an obese group (OG) (C57BL/6J ob/ob) and a control group (CG) (+/+) were used. The tissues were analyzed using a double immunostaining technique for immunoreactivity (ir) of the P2X2 receptor, nitric oxide synthase (NOS), choline acetyl transferase (ChAT) and calretinin (Calr). Also, we investigated the density and profile of neuronal areas of the NOS-, ChAT-and Calr-ir neurons in the myenteric plexus. Myenteric neurons were labeled using an NADH-diaphorase histochemical staining method. RESULTS: The analysis demonstrated that the P2X2 receptor was expressed in the cytoplasm and in the nuclear and cytoplasmic membranes only in the CG. Neuronal density values (neuron/cm(2)) decreased 31% (CG: 6579 +/- 837; OG: 4556 +/- 407) and 16.5% (CG: 7796 +/- 528; OG: 6513 +/- 610) in the NOS-ir and calretinin-ir neurons in the OG, respectively (P < 0.05). Density of ChAT-ir (CG: 6200 +/- 310; OG: 8125 +/- 749) neurons significantly increased 31% in the OG (P < 0.05). Neuron size studies demonstrated that NOS, ChAT, and Calr-ir neurons did not differ significantly between the CG and OG groups. The examination of NADH-diaphorase-positive myenteric neurons revealed an overall similarity between the OG and CG. CONCLUSION: Obesity may exert its effects by promoting a decrease in P2X2 receptor expression and modifications in the density of the NOS-ir, ChAT-ir and CalR-ir myenteric neurons. (C) 2014 Baishideng Publishing Group Inc. All rights reserved. (AU)

• FAPESP's process: 05/04752-0 - Analyses of the distribution and the chemical coding of the P2X2 and P2X7 purine receptors in the ileum and distal colon enteric nervous system of the obese mice
• Grantee: Patricia Castelucci
• Support Opportunities: Regular Research Grants