Highly Sensitive Electrochemical Sensor for Determination of Vitamin D in Mixtures of Water-Ethanol

Author(s)

Cincotto, Fernando H. ^[1] ; Canevari, Thiago C. ^[1] ; Machado, Sergio A. S. ^[1]

Affiliation

^[1] State Univ Sao Paulo, Inst Chem, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Electroanalysis; v. 26, n. 12, p. 2783-2788, DEC 2014.
• Web of Science Citations: 5

Abstract

This paper describes the electrochemical determination of vitamin D-2 (ergocalciferol) and D-3 (cholecalciferol) in mixed organic/water solvent, using a glassy carbon electrode (GCE). The mixing ratio of organic/water solvent has an important influence on the electrocatalytic response of D vitamins on the surface of the glassy carbon electrode. Well-defined peaks for Vitamin D-2 and D-3 were observed in a 40% ethanol/60% water solution with lithium perchlorate as the support electrolyte. This study demonstrated that the glassy carbon electrode is highly sensitive for the determination of vitamin D-2 and D-3, with a limit of detection of 0.13 and 0.118 mu molL(-1), respectively. No significant interference was seen for vitamins A, E and K in the detection of vitamin D. (AU)

• FAPESP's process: 12/18339-1 - Detection of endocrine disruptors testosterone and estradiol in environmental samples utilizing modified electrodes with graphene, the gold nanoparticles and quantum dots.
• Grantee: Fernando Henrique Cincotto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/23047-7 - Development of an electrochemical biosensor based on mesoporous silicon containing carbon nanotubes obtained by a sol-gel process. Electrochemical determination of pesticides.
• Grantee: Thiago da Cruz Canevari
• Support Opportunities: Scholarships in Brazil - Post-Doctoral