Production and Characterization of beta-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31

Author(s)

Pereira, Josiani de Cassia ^[1] ; Ribeiro Leite, Rodrigo Simoes ^[2] ; Alves do Prado, Heloiza Ferreira ^[1] ; Bocchini Martins, Daniela Alonso ^[1] ; Gomes, Eleni ^[1] ; da Silva, Roberto ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Lab Biochem & Appl Microbiol, BR-15054000 Sao Paulo - Brazil

^[2] Fundacao Univ Fed Grande Dourados, FCBA, Biol Environm Sci Fac, BR-79804970 Dourados, MS - Brazil

• Document type: Journal article
• Source: Applied Biochemistry and Biotechnology; v. 175, n. 2, p. 723-732, JAN 2015.
• Web of Science Citations: 7

Abstract

In this paper, several agro-industrial wastes (soybean meal and wheat straw, rice and peanut husks, corn cob and corn stover, and sugarcane bagasse) were tested for the production of beta-glucosidase by the cultivation of thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state fermentation (SSF). Among the tested substrates, the highest yields were obtained in soybean meal. Other fermentation parameters were also evaluated, such as initial pH, merge substrates, and fermentation time, as well as the physicochemical characterization of the enzyme. The best results were obtained after 192 h of fermentation with the initial pH adjusted to 6.0. The substrate mixture did not improve the enzyme production by microorganism. The beta-glucosidase showed best catalytic activity at pH 4.5 and at 75 A degrees C and remained stable in the pH range from 4.5 to 9.5 and the temperature range 40-75 A degrees C. The enzyme showed 80 % of its activity at a concentration of 15 mM glucose and remained stable up to 20 % ethanol. (AU)

• FAPESP's process: 14/02080-4 - Production of ethanol from bagasse sugarcane pretreated with ozone: study of inhibitors, fermentation and industrial yeasts
• Grantee: Roberto da Silva
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 10/12624-0 - Application of physical-chemical and enzymatic methods in the saccharification of sugar cane bagasse: studies on microorganisms, fermentative processes and hydrolyses methods
• Grantee: Eleni Gomes
• Support Opportunities: Research Projects - Thematic Grants