Analysis of cellular adhesion on superhydrophobic and superhydrophilic vertically aligned carbon nanotube scaffolds

Author(s)

Machado, M. M. ^{[1, 2]} ; Lobo, A. O. ^[1] ; Marciano, F. R. ^[1] ; Corat, E. J. ^[3] ; Corat, M. A. F. ^[2]

Affiliation

^[1] Univ Vale Paraiba, Inst Dev Res, Lab Biomed Nanotechnol, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] State Univ Campinas UNICAMP, Multidisciplinary Ctr Biol Invest Lab Anim Sci CE, BR-13083877 Campinas, SP - Brazil

^[3] Natl Inst Space Res INPE, Assoc Lab Sensors & Mat LAS, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 48, p. 365-371, MAR 1 2015.
• Web of Science Citations: 8

Abstract

We analyzed GFP cells after 24 h cultivated on superhydrophilic vertically aligned carbon nanotube scaffolds. We produced two different densities of VACNT scaffolds on Ti using Ni or Fe catalysts. A simple and fast oxygen plasma treatment promoted the superhydrophilicity of them. We used five different substrates, such as: as-grown VACNT produced using Ni as catalyst (Ni), as-grown VACNT produced using Fe as catalyst (Fe), VACNT-O produced using Ni as catalyst (Ni-O), VACNT-O produced using Fe as catalyst (Fe-O) and Ti (control). The 4',6-diamidino-2-phenylindole reagent nuclei stained the adherent cells cultivated on five different analyzed scaffolds. We used fluorescence microscopy for image collect, ImageJ (R) to count adhered cell and GraphPad Prism 5 (R) for statistical analysis. We demonstrated in crescent order: Fe, Ni, Ni-O, Fe-O and Ti scaffolds that had an improved cellular adhesion. Oxygen treatment associated to high VACNT density (group Fe-O) presented significantly superior cell adhesion up to 24 h. However, they do not show significant differences compared with Ti substrates (control). We demonstrated that all the analyzed substrates were nontoxic. Also, we proposed that the density and hydrophilicity influenced the cell adhesion behavior. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
• Grantee: Fernanda Roberta Marciano
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
• Grantee: Anderson de Oliveira Lobo
• Support Opportunities: Research Grants - Young Investigators Grants