Lactobacillus acidophilus ATCC 4356 inhibits biofilm formation by C. albicans and attenuates the experimental candidiasis in Galleria mellonella

Author(s)

Vilela, Simone F. G. ^[1] ; Barbosa, Junia O. ^[1] ; Rossoni, Rodnei D. ^[1] ; Santos, Jessica D. ^[1] ; Prata, Marcia C. A. ^[2] ; Anbinder, Ana Lia ^[1] ; Jorge, Antonio O. C. ^[1] ; Junqueira, Juliana C. ^[1]

Affiliation

^[1] UNESP Univ Estadual Paulista, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos - Brazil

^[2] EMBRAPA, Empresa Brasileira Agr, Juiz De Fora - Brazil

• Document type: Journal article
• Source: VIRULENCE; v. 6, n. 1, p. 29-39, JAN 2015.
• Web of Science Citations: 38

Abstract

Probiotic strains of Lactobacillus have been studied for their inhibitory effects on Candida albicans. However, few studies have investigated the effect of these strains on biofilm formation, filamentation and C. albicans infection. The objective of this study was to evaluate the influence of Lactobacillus acidophilus ATCC 4356 on C. albicans ATCC 18804 using in vitro and in vivo models. In vitro analysis evaluated the effects of L. acidophilus on the biofilm formation and on the capacity of C. albicans filamentation. For in vivo study, Galleria mellonella was used as an infection model to evaluate the effects of L. acidophilus on candidiasis by survival analysis, quantification of C. albicans CFU/mL, and histological analysis. The direct effects of L. acidophilus cells on C. albicans, as well as the indirect effects using only a Lactobacillus culture filtrate, were evaluated in both tests. The in vitro results showed that both L. acidophilus cells and filtrate were able to inhibit C. albicans biofilm formation and filamentation. In the in vivo study, injection of L. acidophilus into G. mellonella larvae infected with C. albicans increased the survival of these animals. Furthermore, the number of C. albicans CFU/mL recovered from the larval hemolymph was lower in the group inoculated with L. acidophilus compared to the control group. In conclusion, L. acidophilus ATCC 4356 inhibited in vitro biofilm formation by C. albicans and protected G. mellonella against experimental candidiasis in vivo. (AU)

• FAPESP's process: 12/02184-9 - Interactions between different Candida species in experimental candidiasis of invertebrates and vertebrates models
• Grantee: Juliana Campos Junqueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/15194-0 - Interactions between different Candida species in experimental candidiasis of invertebrates and vertebrates models
• Grantee: Rodnei Dennis Rossoni
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/19915-6 - Interaction between Streptococcus mutans and Lactobacillus acidophilus with Candida albicans: biofilm formation in vitro and experimental study in invertebrate models
• Grantee: Juliana Campos Junqueira
• Support Opportunities: Regular Research Grants