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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Halistanol sulfate A and rodriguesines A and B are antimicrobial and antibiofilm agents against the cariogenic bacterium Streptococcus mutans

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Author(s):
Lima, Bruna de A. [1, 2] ; de Lira, Simone P. [3] ; Kossuga, Miriam H. [4] ; Goncalves, Reginaldo B. [5] ; Berlinck, Roberto G. S. [4] ; Kamiya, Regianne U. [6]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Faculdade Odontol Piracicaba, Piracicaba, SP - Brazil
[3] Univ Sao Paulo, Escola Super Agron Luiz Queiroz, Piracicaba, SP - Brazil
[4] Univ Sao Paulo, Ins Quim Sao Carlos, Sao Carlos, SP - Brazil
[5] Univ Laval, Fac Med Dent, Quebec City, PQ - Canada
[6] Univ Fed Alagoas, Inst Ciencias Biol & Saude, Maceio, AL - Brazil
Total Affiliations: 6
Document type: Journal article
Source: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 24, n. 6, p. 651-659, NOV-DEC 2014.
Web of Science Citations: 6
Abstract

In the present investigation we report the antibacterial activity of halistanol sulfate A isolated from the sponge Petromica ciocalyptoides, as well as of rodriguesines A and B isolated from the ascidian Didemnum sp., against the caries etiologic agent Streptococcus mutans. The transcription levels of S. mutans virulence genes gtfB, gtfC and gbpB, as well as of housekeeping genes groEL and 16S, were evaluated by sqRT-PCR analysis of S. mutans planktonic cells. There were no alterations in the expression levels of groEL and 16S after antimicrobial treatment with halistanol sulfate A and with rodriguesines A and B, but the expression of the genes gtfB, gtfC and gbpB was down-regulated. Halistanol sulfate A displayed the most potent antimicrobial effect against S. mutans, with inhibition of biofilm formation and reduction of biofilm-associated gene expression in planktonic cells. Halistanol sulfate A also inhibited the initial oral bacteria colonizers, such as Streptococcus sanguinis, but at much higher concentrations. The results obtained indicate that halistanol sulfate A may be considered a potential scaffold for drug development in Streptococcus mutans antibiofilm therapy, the main etiologic agent of human dental caries. (AU)

FAPESP's process: 05/60175-2 - Discovery and development of potential chemotherapeutic agents based on marine invertebrates and associated micro-organisms
Grantee:Roberto Gomes de Souza Berlinck
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 10/50190-2 - Investigation of metabolic and biotechnological potential of marine organisms in bioremediation processes and for the production of substances with anti-viral, anti-inflammatory and anti-Leishmania activities (Marine Biodiversity)
Grantee:Roberto Gomes de Souza Berlinck
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 07/01078-2 - Analysis of antimicrobial activity of purified fractions and extracts of marine invertebrates and microorganisms on Streptococcus mutans and influence on their virulence factors
Grantee:Bruna de Araujo Lima
Support Opportunities: Scholarships in Brazil - Master