Reference values for high-density lipoprotein particle size and volume by dynamic light scattering in a Brazilian population sample and their relationships with metabolic parameters

Author(s):Show less -

Alexandre, F. ^[1] ; Zago, V. H. S. ^[1] ; Panzoldo, N. B. ^[1] ; Parra, E. S. ^[1] ; Scherrer, D. Z. ^[1] ; Vendrame, F. ^[1] ; Nunes, V. S. ^[2] ; Gomes, E. I. L. ^[1] ; Marcato, P. D. ^[3] ; Nakandakare, E. R. ^[2] ; Quintao, E. C. R. ^[2] ; de Faria, E. C. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Med Sci, Dept Clin Pathol, UNICAMP, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Sci, Div Endocrinol & Metab, Lipids Lab LIM 10, Clin Hosp, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribercio Preto, Nanobiolab, Sao Paulo - Brazil

• Document type: Journal article
• Source: Clinica Chimica Acta; v. 442, p. 63-72, MAR 10 2015.
• Web of Science Citations: 1

Abstract

Background: Current data indicate that the size of high-density lipoprotein (HDL) may be considered an important marker for cardiovascular disease risk. We established reference values of mean HDL size and volume in an asymptomatic representative Brazilian population sample (n = 590) and their associations with metabolic parameters by gender. Methods: Size and volume were determined in HDL isolated from plasma by polyethyleneglycol precipitation of apoB-containing lipoproteins and measured using the dynamic light scattering (DLS) technique. Results: Although the gender and age distributions agreed with other studies, the mean HDL size reference value was slightly lower than in some other populations. Both HDL size and volume were influenced by gender and varied according to age. HDL size was associated with age and HDL-C (total population); non- white ethnicity and CETP inversely (females); HDL-C and PLTP mass (males). On the other hand, HDL volume was determined only by HDL-C (total population and in both genders) and by PLTP mass (males). Conclusions: The reference values for mean HDL size and volume using the DLS technique were established in an asymptomatic and representative Brazilian population sample, as well as their related metabolic factors. HDL-C was a major determinant of HDL size and volume, which were differently modulated in females and in males. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 06/60585-9 - Relation of the plasma HDL-cholesterol concentration with blood monocyte and whole body cholesterol metabolism parameters
• Grantee: Eder Carlos Rocha Quintão
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/16476-9 - Physico-chemical and biochemical studies of high density lipoprotein in states of hypo and hyperalphalipoproteinemia: association with carotid artery intima-media thickness
• Grantee: Fernanda Alexandre
• Support Opportunities: Scholarships in Brazil - Master