Chitosan nanoparticles loaded the herbicide paraquat: The influence of the aquatic humic substances on the colloidal stability and toxicity

Author(s):Show less -

Grillo, Renato ^{[1, 2]} ; Clemente, Zaira ^{[2, 3]} ; de Oliveira, Jhones Luis ^[2] ; Ramos Campos, Estefania Vangelie ^{[1, 2]} ; Chalupe, Victor C. ^[3] ; Jonsson, Claudio M. ^[3] ; de Lima, Renata ^[4] ; Sanches, Gabriela ^[4] ; Nishisaka, Caroline S. ^[4] ; Rosa, Andre H. ^[2] ; Oehlke, Kathleen ^[5] ; Greiner, Ralf ^[5] ; Fraceto, Leonardo F. ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem, Campinas, SP - Brazil

^[2] UNESP Univ Eseadual Paulista, Dept Environm Engn, BR-18087180 Sorocaba, SP - Brazil

^[3] Embrapa Environm, Jaguariuna, SP - Brazil

^[4] Univ Sorocaba, Dept Biotechnol, Sorocaba, SP - Brazil

^[5] Max Rubner Inst, Dept Food Technol & Bioproc Engn, Karlsruhe - Germany

• Document type: Journal article
• Source: JOURNAL OF HAZARDOUS MATERIALS; v. 286, p. 562-572, APR 9 2015.
• Web of Science Citations: 17

Abstract

Polymeric nanoparticles have been developed for several applications, among them as carrier system of pesticides. However, few studies have investigated the fate of these materials in the environment in relation to colloidal stability and toxicity. In nature, humic substances are the main agents responsible for complexation with metals and organic compounds, as well as responsible for the dynamics of these nanoparticles in aquatic and terrestrial environments. In this context, the evaluation of the influence of aquatic humic substances (AHS) on the colloidal stability and toxicity of polymeric nanoparticles of chitosan/tripolyphosphate with or without paraquat was performed. In this study, the nanoparticles were prepared by the ionic gelation method and characterized by size distribution measurements (DLS and NTA), zeta potential, infrared and fluorescence spectroscopy. Allium cepa genotoxicity studies and ecotoxicity assays with the alga Pseudokirchneriella subcapitata were used to investigate the effect of aquatic humic substances (AHS) on the toxicity of this delivery system. No changes were observed in the physical-chemical stability of the nanoparticles due to the presence of AHS using DLS and NTA techniques. However some evidence of interaction between the nanoparticles and AHS was observed by infrared and fluorescence spectroscopies. The ecotoxicity and genotoxicity assays showed that humic substances can decrease the toxic effects of nanoparticles containing paraquat. These results are interesting because they are important for understanding the interaction of these nanostructured carrier systems with species present in aquatic ecosystems such as humic substances, and in this way, opening new perspectives for studies on the dynamics of these carrier systems in the ecosystem. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/12322-2 - Nanoparticles as carrier systems for bioactive compounds aiming agriculture applications
• Grantee: Leonardo Fernandes Fraceto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/01872-6 - Polymeric nanoparticles as delivery system for herbicides: preparation, characterization, interaction with humic substances and assessment of biological activity
• Grantee: Renato Grillo
• Support Opportunities: Scholarships in Brazil - Doctorate