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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Thermophilic fungi as new sources for production of cellulases and xylanases with potential use in sugarcane bagasse saccharification

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Author(s):
de Cassia Pereira, J. [1] ; Paganini Marques, N. [2] ; Rodrigues, A. [3] ; Brito de Oliveira, T. [3] ; Boscolo, M. [4] ; da Silva, R. [1] ; Gomes, E. [1] ; Bocchini Martins, D. A. [2]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP IBILCE, Lab Biochem & Appl Microbiol, Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP IQ, Lab Microbial Enzymes, Sao Paulo - Brazil
[3] Sao Paulo State Univ IB UNESP, Lab Ecol & Systemat Fungi, Sao Paulo - Brazil
[4] Sao Paulo State Univ IB UNESP, Lab Sucrochem & Analyt Chem, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Applied Microbiology; v. 118, n. 4, p. 928-939, APR 2015.
Web of Science Citations: 29
Abstract

AimsTo obtain new cellulases and xylanases from thermophilic fungi; evaluate their potential for sugarcane bagasse saccharification. Methods and ResultsThirty-two heat-tolerant fungi were isolated from the environment, identified (morphological/molecular tools) and the production of the enzymes was evaluated by solid state fermentation using lignocellulosic materials as substrates. Myceliophthora thermophila JCP 1-4 was the best producer of endoglucanase (35751Ug(-1)), -glucosidase (4542Ug(-1)), xylanase (93111Ug(-1)) and avicelase (358Ug(-1)). These enzymes were most active at 55-70 degrees C and stable at 30-60 degrees C. Using crude enzymatic extract from M.thermophila JCP 1-4 to saccharify sugarcane bagasse pretreated with microwaves and glycerol, glucose and xylose yields obtained were 156 and 3513% (22 and 195g l(-1)), respectively. ConclusionsAll isolated fungi have potential to produce the enzymes; M.thermophila JCP 1-4 enzymatic extract have potential to be better explored in saccharification experiments. Pretreatment improved enzymatic saccharification, as sugar yields were much higher than those obtained from in natura bagasse. Significance and Impact of the StudyMyceliophthora thermophila JCP 1-4 produces avicelase (not commonly found among fungi; important to hydrolyse crystalline cellulose) and a -glucosidase resistant to glucose inhibition, interesting characteristics for saccharification experiments. (AU)

FAPESP's process: 14/02080-4 - Production of ethanol from bagasse sugarcane pretreated with ozone: study of inhibitors, fermentation and industrial yeasts
Grantee:Roberto da Silva
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 10/12624-0 - Application of physical-chemical and enzymatic methods in the saccharification of sugar cane bagasse: studies on microorganisms, fermentative processes and hydrolyses methods
Grantee:Eleni Gomes
Support Opportunities: Research Projects - Thematic Grants