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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microsatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technology

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Author(s):
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Mendes, N. J. [1] ; Cruz, V. P. [1] ; Mendonca, Fernando Fernandes [1, 2] ; Pardo, B. G. [3] ; Coelho, R. [4, 5] ; Ashikaga, F. Y. [1] ; Camargo, S. M. [1, 2] ; Martinez, P. [3] ; Oliveira, C. [1] ; Santos, M. N. [4] ; Foresti, F. [1]
Total Authors: 11
Affiliation:
[1] UNESP, Lab Biol & Genet Peixes, Inst Biociencias, BR-18618970 Botucatu, SP - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Inst Mar, Lab Genet Pesqueira & Conservac, BR-11030400 Santos, SP - Brazil
[3] Univ Santiago de Compostela, Fac Vet, Dept Genet, Lugo 27002 - Spain
[4] IPMA IP, Inst Portugues Mar & Atmosfera, Olhao - Portugal
[5] Univ Algarve, CCMAR, Ctr Ciencias Mar, Faro - Portugal
Total Affiliations: 5
Document type: Journal article
Source: CONSERVATION GENETICS RESOURCES; v. 7, n. 2, p. 585-589, JUN 2015.
Web of Science Citations: 0
Abstract

Among the pelagic shark species most impacted by fisheries, the oceanic whitetip shark (Carcharhinus longimanus) has shown signs of population declines. In this study, we developed 12 microsatellite markers using next generation sequencing (454 pyrosequencing) in C. longimanus for further use in population screening. The genetic diversity of these loci was assessed in 28 individuals and a total of 35 alleles were identified, ranging from four to eight alleles per locus (5.9 alleles on average). Cross-species amplification of these markers was successful in five loci for C. acronotus, C. perezi and Galeocerdo cuvier. The developed microsatellite markers will be useful in analyzing the population genetics structure of the oceanic whitetip shark and other Carcharhinidae species. (AU)

FAPESP's process: 10/51903-2 - Phylogeography of pelagic sharks in the Atlantic Ocean using molecular markers
Grantee:Fausto Foresti
Support Opportunities: Regular Research Grants
FAPESP's process: 13/14555-4 - Development of microsatellite markers for sharks Carcharhinus acronotus and Galeocerdo cuvier using second generation sequencing
Grantee:Natália Jade Mendes
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 11/23787-0 - Molecular biodiversity and conservation of sharks: gene bank, population structure and international scientific network of collaboration
Grantee:Fernando Fernandes Mendonça
Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants