Isolation and characterisation of microsatellite markers for Centrolobium tomentosum (Fabaceae), a neotropical tree species widely used for Atlantic Rainforest restoration

Author(s)

Sujii, Patricia Sanae ^[1] ; Schwarcz, Kaiser Dias ^[1] ; Grando, Carolina ^[1] ; do Valle, Giuliana Etore ^[2] ; de Campos, Jaqueline Bueno ^[1] ; Pinheiro, Jose Baldin ^[3] ; Zucchi, Maria Imaculada ^[4]

Affiliation

^[1] Univ Estadual Campinas, Dept Genet Evolucao & Bioagentes, Campinas - Brazil

^[2] Inst Agronom Campinas, Ctr Fitossanidade Entomol, Campinas, SP - Brazil

^[3] ESALQ USP, Dept Genet, Piracicaba - Brazil

^[4] Agencia Paulista Tecnol Agronegocios, Polo Ctr Sul, Piracicaba, SP - Brazil

• Document type: Journal article
• Source: CONSERVATION GENETICS RESOURCES; v. 7, n. 3, p. 733-734, SEP 2015.
• Web of Science Citations: 2

Abstract

We isolated and characterised eight pairs of primers to amplify microsatellite regions for Centrolobium tomentosum, a neotropical tree species widely used for forest restoration, with important pharmacological potential. For the primer characterisation, we genotyped 48 individuals from two populations of C. tomentosum from natural remnants of Atlantic Rainforests. We detected 2-9 alleles per locus, observed and expected heterozygosities ranged from 0.08 to 0.72, and 0.08 to 0.83, respectively and we observed private alleles in six of the loci. No linkage disequilibrium was observed and all loci are in Hardy-Weinberg Equilibrium in at least one of the populations. This study presents a powerful tool for population genetic studies of this species. (AU)

• FAPESP's process: 11/50296-8 - Conservation biology of Atlantic Forest’s medicinal species: a genetical approach to Forest restorations
• Grantee: Maria Imaculada Zucchi
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 12/03246-8 - Genetic diversity and structure and reproductive system in Centrolobium tomentosum Guillem. Bentham (Fabaceae) aiming to support genetic enrichment in areas of forest restoration
• Grantee: Patricia Sanae Sujii
• Support Opportunities: Scholarships in Brazil - Doctorate