Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content

Author(s)

Vicentini, Renato ^[1] ; Bottcher, Alexandra ^[2] ; Brito, Michael dos Santos ^{[2, 3]} ; dos Santos, Adriana Brombini ^[2] ; Creste, Silvana ^[3] ; de Andrade Landell, Marcos Guimaraes ^[3] ; Cesarino, Igor ^[4] ; Mazzafera, Paulo ^[2]

Affiliation

^[1] Univ Estadual Campinas, Ctr Mol Biol & Genet Engn, Syst Biol Lab, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil

^[3] Agron Inst Campinas, Sugarcane Ctr, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Inst Biosci, Dept Bot, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 10, n. 8 AUG 4 2015.
• Web of Science Citations: 22

Abstract

Sugarcane is an important crop worldwide for sugar and first generation ethanol production. Recently, the residue of sugarcane mills, named bagasse, has been considered a promising lignocellulosic biomass to produce the second-generation ethanol. Lignin is a major factor limiting the use of bagasse and other plant lignocellulosic materials to produce second-generation ethanol. Lignin biosynthesis pathway is a complex network and changes in the expression of genes of this pathway have in general led to diverse and undesirable impacts on plant structure and physiology. Despite its economic importance, sugarcane genome was still not sequenced. In this study a high-throughput transcriptome evaluation of two sugarcane genotypes contrasting for lignin content was carried out. We generated a set of 85,151 transcripts of sugarcane using RNA-seq and de novo assembling. More than 2,000 transcripts showed differential expression between the genotypes, including several genes involved in the lignin biosynthetic pathway. This information can give valuable knowledge on the lignin biosynthesis and its interactions with other metabolic pathways in the complex sugarcane genome. (AU)

• FAPESP's process: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
• Grantee: Paulo Mazzafera
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 08/58031-0 - Sugarcane energetic balance: a systems approach towards understanding regulation of sucrose metabolism and sugar signaling
• Grantee: Renato Vicentini dos Santos
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants