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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells

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Author(s):
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Oliveira-Costa, Joao Paulo [1] ; de Carvalho, Alex Fiorini [2] ; da Silveira, Giorgia Gobbi [1] ; Amaya, Peter [3] ; Wu, Yongqi [3] ; Park, Kyoung-Joo Jenny [3] ; Gigliola, Mabel Pinilla [2] ; Lustberg, Maryam [4] ; Cavicchioli Buim, Marcilei Eliza [5] ; Ferreira, Elisa Napolitano [2] ; Kowalski, Luiz Paulo [6] ; Chalmers, Jeffrey J. [3] ; Soares, Fernando Augusto [5] ; Carraro, Dirce Maria [2] ; Ribeiro-Silva, Alfredo [1]
Total Authors: 15
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Pathol, BR-14049 Ribeirao Preto - Brazil
[2] AC Camargo Canc Ctr, CIPE, Lab Genom & Mol Biol, Sao Paulo - Brazil
[3] Ohio State Univ, William G Lowrie Dept Chem & Biomol Engn, Columbus, OH 43210 - USA
[4] Ohio State Univ, Wexner Med Ctr, Stefanie Spielman Comprehens Breast Ctr, Columbus, OH 43210 - USA
[5] AC Camargo Canc Ctr, Dept Anat Pathol, Sao Paulo - Brazil
[6] AC Camargo Canc Ctr, Dept Head & Neck Surg & Otorhinolaryngol, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: ONCOTARGET; v. 6, n. 25, p. 20902-20920, AUG 28 2015.
Web of Science Citations: 40
Abstract

Oral squamous cell carcinoma (OSCC) is the most common tumor of the oral cavity and has been associated with poor prognosis. Scarce prognostic markers are available for guiding treatment and/or sub-classifying patients. This study aims to identify biomarkers by searching for genes whose expression is increased or decreased during tumor progression (through T1 to T4 stages). Thirty-six samples from all tumor size stages (from T1 to T4) were analyzed using cDNA microarrays. Selected targets were analyzed by immunohistochemistry and in circulating tumor cells by immunofluorescence and Nanostring. Correlation was shown between PD-L1 and tumor size and lymph node metastasis, HOXB9 and tumor size, BLNK and perineural invasion, and between ZNF813 and perineural invasion. PD-L1 positivity was an independent prognostic factor in this cohort (p = 0.044, HH = 0.426). In CTCs from patients with locally advanced OSCC, we found a strong cytoplasmatic expression of PD-L1. PD-L1 is a ligand of PD-1 and is believed to limit T cell activity in inflammatory responses and limit autoimmune diseases. We demonstrated an important role for PD-L1 in primary tumors according to tumor size, and in disease specific survival. Therefore, we could further determine individuals with PD-L1+ CTCs, and possibly follow treatment using CTCs. (AU)

FAPESP's process: 14/14991-1 - Fibroblast growth factor receptors status in squamous cell carcinomas and circulating tumor cells
Grantee:João Paulo Oliveira da Costa
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/08400-0 - Identification of molecular profiles associated to the development of oral squamous cell carcinoma using the cDNA microarrays technology
Grantee:João Paulo Oliveira da Costa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/08637-0 - Identification of molecular profiles associated to the development of oral squamous cell carcinoma using the cDNA microarray technology
Grantee:Alfredo Ribeiro da Silva
Support type: Regular Research Grants
FAPESP's process: 11/18606-7 - CDNA and lincRNA microarrays: identification of expression patterns related to metastatization in oral squamous cell carcinoma
Grantee:Fernando Augusto Soares
Support type: Regular Research Grants