Determination of biogenic amines in beer and wine by capillary electrophoresis-tandem mass spectrometry

Author(s)

Daniel, Daniela ^{[1, 2]} ; dos Santos, Vagner Bezerra ^[1] ; Rajh Vidal, Denis Tadeu ^[1] ; do Lago, Claudimir Lucio ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508000 Sao Paulo, SP - Brazil

^[2] Agilent Technol, BR-06455000 Barueri, SP - Brazil

• Document type: Journal article
• Source: Journal of Chromatography A; v. 1416, p. 121-128, OCT 16 2015.
• Web of Science Citations: 49

Abstract

A capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) method for the simultaneous assessment of nine biogenic amines (spermine, spermidine, putrescine, cadaverine, histamine, phenylethylamine, tryptamine, tyramine, and urocanic acid) in commercial samples of beer and wine is introduced. The samples were submitted to a simple clean-up step with poly(vinylpolypyrrolidone) followed by filtration. Electrophoretic separation in a polyvinyl alcohol (PVA)-coated capillary using 0.5 mol L-1 acetic acid (pH 2.5) as background electrolyte and detection by electrospray-tandem mass spectrometry was employed. The range of the correlation coefficients of the calibration curves of the analyzed compounds was 0.996-0.999, and the limits of detection and limits of quantification were in the range of 1-2 mu g L-1 and 3-8 mu g L-1, respectively. The recovery values for samples spiked at three concentration levels (0.2, 0.5, and 1.0 mg L-1) ranged from 87 to 113% with standard deviation not greater than 5.8%. The use of a PVA-coated silica capillary allows suppressing the electroosmotic flow and, consequently, increasing of the separation efficiency. The method was successfully used to determine biogenic amines in commercial samples of beer and wine. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/02156-2 - The use of capillary electrophoresis with contactless conductivity detection for obtaining analytical and physicochemical parameters of peroxycarbonate, peroxyacids, and monoethyl pyrocarbonate.
• Grantee: Denis Tadeu Rajh Vidal
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/14993-1 - Characterization and quantification of monoalkyl carbonates in aqueous medium employing microfluidic devices coupled to a mass spectrometer
• Grantee: Vagner Bezerra dos Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/06642-1 - Formation and properties of adducts of organic hydroxy-compounds with carbonate in aqueous medium and biological conditions
• Grantee: Claudimir Lucio Do Lago
• Support Opportunities: Regular Research Grants