Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Environmentally Relevant Concentrations of Atrazine and Ametrine Induce Micronuclei Formation and Nuclear Abnormalities in Erythrocytes of Fish

Full text
Author(s):
Botelho, R. G. [1] ; Monteiro, S. H. [2] ; Christofoletti, C. A. [3] ; Moura-Andrade, G. C. R. [1] ; Tornisielo, V. L. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo CENA USP, Lab Ecotoxicol Aquat, Ctr Energia Nucl Agr, BR-13416000 Piracicaba, SP - Brazil
[2] Ctr Pequisa & Desenvolvimento Protecao Ambiental, Inst Biol, BR-04014002 Sao Paulo, SP - Brazil
[3] Ctr Univ Herminio Ometto FHO UNIARARAS, BR-13607339 Araras, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Archives of Environmental Contamination and Toxicology; v. 69, n. 4, p. 577-585, NOV 2015.
Web of Science Citations: 8
Abstract

A rapid and sensitive method using liquid chromatography coupled with mass spectrometry triple quadrupole direct aqueous injection for analysis of atrazine and ametrine herbicides in surface waters was developed. According to the validation method, water samples from six different locations in the Piracicaba River were collected monthly from February 2011 to January 2012 and injected into a liquid chromatographer/dual mass spectrometer without the need for sample extraction. The method was validated and shown to be precise and accurate; limits of detection and quantification were 0.07 and 0.10 A mu g L-1 for atrazine and 0.09 and 0.14 A mu g L-1 for ametrine. During the sampling period, concentrations of atrazine ranged from 0.11 to 1.92 A mu g L-1 and ametrine from 0.25 to 1.44 A mu g L-1. After analysis of the herbicides, Danio rerio were exposed a range of concentrations found in the river water to check the induction of micronuclei and nuclear abnormalities (NAs) in erythrocytes. Concentrations of atrazine and ametrine > 1.0 and 1.5 A mu g L-1, respectively, induced MN formation in D. rerio. Ametrine was shown to be more genotoxic to D. rerio because a greater incidence of NAs was observed compared with atrazine. Therefore, environmentally relevant concentrations of atrazine and ametrine found in the Piracicaba River are dangerous to the aquatic biota. (AU)

FAPESP's process: 10/18895-6 - Determination of water quality in the Piracicaba River through analysis ecotoxicological and physico-chemical
Grantee:Valdemar Luiz Tornisielo
Support Opportunities: Regular Research Grants
FAPESP's process: 10/04756-4 - Assessment of water quality of the Piracicaba River (São Paulo, Brazil) and effects of vinasse to aquatic organisms before and after pH correction
Grantee:Rafael Grossi Botelho
Support Opportunities: Scholarships in Brazil - Doctorate