Arginase inhibition, antibacterial and antioxidant activities of Pitanga seed (Eugenia uniflora L.) extracts from sustainable technologies of high pressure extraction

Author(s)

Santos, Debora Nascimentoe ^[1] ; de Souza, Larissa Lima ^[1] ; Fernandes de Oliveira, Carlos Augusto ^[1] ; Silva, Edson Roberto ^[2] ; de Oliveira, Alessandra Lopes ^[1]

Affiliation

^[1] Univ Sao Paulo, FZEA, Dept Engn Alimentos, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, FZEA, Dept Med Vet, BR-13635900 Pirassununga, SP - Brazil

• Document type: Journal article
• Source: FOOD BIOSCIENCE; v. 12, p. 93-99, DEC 1 2015.
• Web of Science Citations: 4

Abstract

Pitanga seed extracts from supercritical extraction (SFE), pressurized liquid extraction (PLE) and conventional Soxhlet extraction had their bioactive properties tested in vitro. The essays included antioxidant (reducing power, DPPH center dot and ABTS(center dot+)) and antibacterial (S. aureus, E. coil, P. aeruginosa and B. subtilis) activities, as well as the arginase enzyme inhibition test. Purified PLE extracts presented the highest antioxidant capacity (33.37 mu M Trolox x g(-1) extract) and arginase inhibition (75.96%). All extracts presented the same value of minimum concentration inhibition, 125 mu g x g(-1), indicating high antibacterial potential. The purified fractions from extracts obtained using SFE and PLE processes had increased arginase from Leishmania inhibition, 48.48% and 75.96% respectively, compared to Soxhlet (37.59%). These findings suggest that using different extraction methods on Pitanga seed can produce compounds with diverse activities. Extracts from Pitanga seed, a residue from the juice processing industry, possess characteristic fruit volatiles and could be used as natural aroma enrichment of Pitanga products or in functional foods formulations since compounds of the extracts present bioactive activities. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 08/00148-0 - Collection and analysis of extracts from seeds of pitanga (Eugenia uniflora L.) obtained by use of supercritical carbon dioxide
• Grantee: Alessandra Lopes de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/16665-3 - Green coffee oil extraction by supercritical and pressurized fluid process, and comparison with the composition of coffee oil obtained by pressing for the small producers of the State of São Paulo - concentration analysis of bioactive diterpenes
• Grantee: Alessandra Lopes de Oliveira
• Support Opportunities: Regular Research Grants