Impact of aging on cardiac function in a female rat model of menopause: role of autonomic control, inflammation, and oxidative stress

Author(s):Show less -

Machi, Jacqueline Freire ^{[1, 2]} ; Dias, Danielle da Silva ^[3] ; Freitas, Sarah Cristina ^[3] ; de Moraes, Oscar Albuquerque ^[2] ; da Silva, Maikon Barbosa ^[2] ; Cruz, Paula Lazara ^[2] ; Mostarda, Cristiano ^[4] ; Salemi, Vera M. C. ^[2] ; Morris, Mariana ^[1] ; De Angelis, Katia ^[3] ; Irigoyen, Maria-Claudia ^[2]

Affiliation

^[1] Nova SE Univ, Inst Neuroimmune Med, Ft Lauderdale, FL 33314 - USA

^[2] Univ Sao Paulo, Sch Med, Hypertens Unit, Heart Inst InCor, Ave Dr Eneas de Carvalho Aguiar 44, BR-05403900 Sao Paulo, SP - Brazil

^[3] Univ Nove Julho UNINOVE, Lab Translat Physiol, Sao Paulo - Brazil

^[4] Fed Univ Maranhao UFMA, Hlth Adult & Child, Sao Luiz Maranhao - Brazil

• Document type: Journal article
• Source: CLINICAL INTERVENTIONS IN AGING; v. 11, p. 341-350, 2016.
• Web of Science Citations: 13

Abstract

Objective: The aim of this study was to evaluate the effects of aging on metabolic, cardiovascular, autonomic, inflammatory, and oxidative stress parameters after ovarian hormone deprivation (OVX). Methods: Female Wistar rats (3 or 22 months old) were divided into: young controls, young ovariectomized, old controls, and old ovariectomized (bilateral ovaries removal). After a 9-week follow-up, physical capacity, metabolic parameters, and morphometric and cardiac functions were assessed. Subsequently, arterial pressure was recorded and cardiac autonomic control was evaluated. Oxidative stress was measured on the cardiac tissue, while inflammatory profile was assessed in the plasma. Results: Aging or OVX caused an increase in body and fat weight and triglyceride concentration and a decrease in both insulin sensitivity and aerobic exercise capacity. Left ventricular diastolic dysfunction and increased cardiac overload (myocardial performance index) were reported in old groups when compared with young groups. Aging and OVX led to an increased sympathetic tonus, and vagal tonus was lower only for the old groups. Tumor necrosis factor-a and interleukin-6 were increased in old groups when compared with young groups. Glutathione redox balance (GSH/GSSG) was reduced in young ovariectomized, old controls, and old ovariectomized groups when compared with young controls, indicating an increased oxidative stress. A negative correlation was found between GSH/GSSG and tumor necrosis factor-a (r=-0.6, P<0.003). Correlations were found between interleukin-6 with adipose tissue (r=0.5, P<0.009) and vagal tonus (r=-0.7, P<0.0002); and among myocardial performance index with interleukin-6 (r=0.65, P<0.0002), sympathetic tonus (r=0.55, P<0.006), and physical capacity (r=-0.55, P<0.003). The findings in this trial showed that ovariectomy aggravated the impairment of cardiac and functional effects of aging in female rats, probably associated with exacerbated autonomic dysfunction, inflammation, and oxidative stress. (AU)

• FAPESP's process: 12/20141-5 - Effects of aerobic,resistance or combined exercise training in experimental models of cardiometabolic disorders associated with ovarian hormone deprivation
• Grantee: Kátia de Angelis Lobo D Avila
• Support Opportunities: Regular Research Grants