Feasibility of Using AAS for the Characterization of a Tuna Fish Candidate Reference Material for Total Hg and Methyl-Hg Measurement

Author(s)

Chelegao, Rodrigo ^[1] ; Carioni, Vivian M. O. ^[2] ; Naozuka, Juliana ^[3] ; Nomura, Cassiana S. ^[2]

Affiliation

^[1] Embrapa Agrossilvipastoril, Ctr Pesquisas Agr, BR-78550970 Sinop, MT - Brazil

^[2] Univ Sao Paulo, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP - Brazil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 27, n. 4, p. 712-718, APR 2016.
• Web of Science Citations: 3

Abstract

This work describes the evaluation of several parameters for preparing a tuna fish candidate reference material (RM) in order to measure its total Hg and methyl-Hg mass fractions by cold vapor atomic absorption spectrometry (CV-AAS) and solid sampling graphite furnace atomic absorption spectrometry (SS-GF AAS). Best results were acquired after extraction with toluene and L-cysteine for 15 min at 80 °C. The parameters investigated were stability of chemical composition during production, homogeneity and analyte segregation. No analyte segregation was observed and the material was shown to be stable even when stored at 50 °C for 10 months. Total Hg mass fraction was 3.03 ± 0.22 µg g-1 by CV AAS, 2.99 ± 0.43 µg g-1 by SS-GF AAS and 2.95 ± 0.15 µg g-1 by direct mercury analyser (DMA). Methyl-Hg mass fraction was 2.49 ± 0.13 µg g-1 by CV AAS and 2.44 ± 0.46 µg g-1 by SS-GF AAS. (AU)

• FAPESP's process: 12/11998-0 - Evaluation and production of synthetic calibrating material for direct analysis of wood by LIBS
• Grantee: Cassiana Seimi Nomura
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/11517-1 - Domestic cooking effects of beans (Phaseolus vulgaris L.) in the total concentration of essential elements and in the distribution of proteins and Cu and Fe species
• Grantee: Juliana Naozuka
• Support Opportunities: Regular Research Grants