Cell growth on 3D microstructured surfaces

Author(s)

Araujo, W. W. R. ^[1] ; Teixeira, F. S. ^[1] ; da Silva, G. N. ^[2] ; Salvadori, D. M. F. ^[3] ; Salvadori, M. C. ^[1] ; Brown, I. G. ^[4]

Affiliation

^[1] Univ Sao Paulo, Inst Phys, CP 66318, BR-05315970 Sao Paulo, SP - Brazil

^[2] UFOP Fed Univ Ouro Preto, Sch Pharm, Ouro Preto, MG - Brazil

^[3] UNESP Sao Paulo State Univ, Botucatu Med Sch, Botucatu, SP - Brazil

^[4] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, 1 Cyclotron Rd, Berkeley, CA 94720 - USA

• Document type: Journal article
• Source: Materials Science & Engineering C-Materials for Biological Applications; v. 63, p. 686-689, JUN 1 2016.
• Web of Science Citations: 2

Abstract

Chinese Hamster Ovary (CHO) cell cultures were grown on surfaces lithographed with periodic 3D hexagonal microcavity array morphology. The range of microcavity size (inscribed circle diameter) was from 12 mu m to 560 mu m. CHO cells were grown also on flat surfaces. The characterization was performed with respect to cell growth density (number of nuclei per unit area) by fluorescence optical microscopy and evaluated by correlation function analysis. We found that optimum microcavity radius was 80 mu m, concerning to the maximum cell growth density, being even greater than the growth density on a flat (unstructured) substrate of the same material. This finding can be important for optimization of biotechnological processes and devices. (C) 2016 Published by Elsevier B.V. (AU)

• FAPESP's process: 11/00624-9 - Development of a novel kind of ion implanter and its application in surfaces modification
• Grantee: Maria Cecília Barbosa da Silveira Salvadori
• Support Opportunities: Regular Research Grants