Attenuated allergic inflammatory response in the lungs during lactation

Author(s):Show less -

Ochoa-Amaya, Julieta E. ^{[1, 2]} ; Marino, Larissa P. ^[3] ; Tobaruela, Carla N. ^[1] ; Namazu, Lilian B. ^[1] ; Calefi, Atilio S. ^[1] ; Margatho, Rafael ^[1] ; Goncalves, Jr., Vagner ^[1] ; Queiroz-Hazarbassanov, Nicolle ^[1] ; Klein, Marianne O. ^[1] ; Palermo-Neto, Joao ^[1] ; Ligeiro de Oliveira, Ana P. ^[4] ; Massoco, Cristina de O. ^[1] ; Felicio, Luciano F. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Patol, Fac Med Vet, Sao Paulo, SP - Brazil

^[2] Univ Llanos, Programa Med Vet & Zootecnia, Fac Ciencias Agr & Recursos Nat, Villavicencio - Colombia

^[3] Univ Santo Amaro, Sao Paulo, SP - Brazil

^[4] Univ Nove de Julho, Programa Posgrad Biofoton Aplicada Ciencias Saude, Sao Paulo - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 151, p. 281-287, APR 15 2016.
• Web of Science Citations: 2

Abstract

Aims: To evaluate the influence of lactation on lung immune function during allergic inflammation. Main methods: Female rats, 60-90 days old, were divided into three groups: no lung allergy virgins (N group), ovalbumin (OVA)-immunized and sensitized virgins (V group), and OVA-immunized and sensitized lactating females (L group). On gestation day (GD) 10, all animals in L group received a subcutaneous injection of 0.1 mg.kg(-1) OVA plus aluminum hydroxide. On GD17, the L group received a subcutaneous booster injection of 10 mu g OVA plus 10 mg aluminum hydroxide. After 7 days, an inhalatory challenge with 1% OVA was given in 15 min sessions for 3 consecutive days. Animals from the V group received the same treatment, meaning both tests and time intervals between OVA treatment and inhalatory challenge were the same as in the L group. Twenty-four hours after the last inhalation session, the animals were euthanized, and the following tests were performed: total and differential bronchoalveolar lavage (BAL) and femoral marrow lavage (FML) leukocyte counts, quantification of tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) levels in BAL fluid, and quantification of plasma corticosterone and catecholamine levels. Key findings: The L group presented lower BAL total leukocyte counts and decreases in the number of eosinophils and macrophages compared with the V group. They also expressed higher BAL IFN-gamma and lower plasma corticosterone levels. Plasma norepinephrine levels were higher in the L group than in the N and V groups. Significance: Lactating female rats presented less intense allergic lung inflammation. Our findings suggest that lactation may protect females from asthmatic crises. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 13/01610-7 - Subsistence and reproduction: opioidergic modulation of behavioral selection during lactation
• Grantee: Luciano Freitas Felicio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/51886-3 - Neuroimmunomodulation: drugs, stress and cytokines on nervous, endocrine and immune systems relationships
• Grantee: João Palermo Neto
• Support Opportunities: Research Projects - Thematic Grants