Antimicrobial potential of Casearia sylvestris against oral bacteria

Author(s)

Amanda Henriques CAVALHEIRO ^[1] ; Ana Paula Terossi GODOI ^[2] ; Tatiane Cruz de CARVALHO ^[3] ; Alma Blasida CATIRSE ^[4] ; Niege Araçari Jacometti Cardoso FURTADO ^[5]

Affiliation

^[1] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Brasil

^[2] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto - Brasil

^[3] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Brasil

^[4] Universidade de São Paulo. Faculdade de Odontologia de Ribeirão Preto - Brasil

^[5] Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Brasil

• Document type: Journal article
• Source: Rev. odontol. UNESP; v. 45, n. 4, p. 214-218, 2016-08-04.

Abstract

Abstract Aim The aim of this study was to obtain Casearia sylvestris leave extracts by different extractive methods, including the obtention of essential oil, in order to compare their antimicrobial activities to conventional mouthwash chlorhexidine against oral bacteria. Material and method For this evaluation, extracts from the leaves were obtained by different methods of extraction (infusion, decoction, maceration and percolation) using different solvent systems: water 100%, ethanol 100%, methanol 100%, water: ethanol 3:7; water: ethanol 7:3; water: methanol 7:3 and water: methanol 3:7. The essential oil, which corresponds to a volatile fraction, was obtained by hydrodistillation using Clevenger modified apparatus. The microdilution broth method was used to determine the values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the following microorganisms: Streptococcus mutans ATCC 25175, S. mitis ATCC 49456, S. sanguinis ATCC 10556, S. salivarius ATCC 25975, Lactobacillus casei ATCC 11578 and Enterococcus faecalis ATCC4082. Chlorhexidine gluconate was used as a positive control. Result All extracts evaluated in the used protocol displayed MIC values higher than 400 µg/mL and few showed bactericidal activity. The antimicrobial activity of essential oil was higher than the activity of the extracts, and the best minimum inhibitory concentration and minimum bactericidal concentration values were obtained against L. casei (MIC of 0.023 µg/mL and MBC of 0.046 µg/mL) and S. mutans (MIC of 25 µg/mL and MBC of 50 µg/mL), respectively. Conclusion The essential oil of Casearia sylvestris has significant antimicrobial activity against oral microorganisms. (AU)

• FAPESP's process: 11/23866-8 - Cytotoxicity and antimicrobial potential of essential oil of Casearia Sylvestris and effect of mouthwash formulation with bioactive essential oil on properties of dental restorative materials.
• Grantee: Ana Paula Terossi de Godoi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/08185-7 - Cytotoxicity and antimicrobial potential of essential oil of Casearia sylvestris and effect of mouthwash formulation with bioactive essential oil on properties of dental restorative materials
• Grantee: ALMA BLASIDA CONCEPCION ELIZAUR BENITEZ CATIRSE
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/07328-6 - Evaluation of antimicrobial potential of Casearia sylvestris against microorganisms from dental caries and study of the effect of formulation of mouthwash with bioactive extract on the color of dental restorative materials
• Grantee: Amanda Henriques Cavalheiro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation