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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rapid Detection of ACTG- and AK-Toxins in Alternaria alternata by LC-ESI-MS/MS Analysis and Antifungal Properties of Citrus Compounds

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Author(s):
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Kátia R. Prieto [1] ; Lívia S. de Medeiros [2] ; Marsele M. Isidoro [3] ; Leonardo Toffano [4] ; Maria Fátima G. F. da Silva [5] ; João B. Fernandes [6] ; Paulo C. Vieira [7] ; Moacir R. Forim [8] ; Edson Rodrigues-Filho [9] ; Rodrigo M. Stuart [10] ; Marcos A. Machado [11]
Total Authors: 11
Affiliation:
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[1] Universidade Federal de São Carlos. Departamento de Química - Brasil
[2] Universidade Federal de São Carlos. Departamento de Química - Brasil
[3] Universidade Federal de São Carlos. Departamento de Química - Brasil
[4] Universidade Federal de São Carlos. Departamento de Química - Brasil
[5] Universidade Federal de São Carlos. Departamento de Química - Brasil
[6] Universidade Federal de São Carlos. Departamento de Química - Brasil
[7] Universidade Federal de São Carlos. Departamento de Química - Brasil
[8] Universidade Federal de São Carlos. Departamento de Química - Brasil
[9] Universidade Federal de São Carlos. Departamento de Química - Brasil
[10] Instituto Agronômico. Centro APTA Citros Sylvio Moreira - Brasil
[11] Instituto Agronômico. Centro APTA Citros Sylvio Moreira - Brasil
Total Affiliations: 11
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 27, n. 8, p. 1493-1505, 2016-08-00.
Abstract

A methanol-soluble fraction of the dichloromethane extract from the culture broth of A. alternata AT4303 strain afforded the following five known mycotoxins: alternariol, alternariol monomethyl ether, altenusin, altenuene and altertoxin I. A hexane-soluble fraction of the dichloromethane extract yielded the steroids ergosterol and ergosterol peroxide, and alternariol monomethyl ether. The concentrated ethyl acetate extract afforded the nucleoside uridine, uracil and inosine. The micro-extracts obtained from this strain in solid media were analyzed using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), and the following five known toxins were identified: ACTG-C, D, E and F and AK-toxin II. The major toxins produced by the tangerine pathotype ACT-toxins appear to be absent in strain AT4303. However, the low concentration of ACT-toxins appears to be responsible for their lack of detection in this study. Some compounds isolated from citrus plants were tested for in vitro activity against this strain. The results indicated that 17.3 µM apigenin-7-O-rutinoside inhibited by 80% conidial germination and appressorium development. (AU)

FAPESP's process: 12/25299-6 - Integrated studies for leaf cutting control
Grantee:João Batista Fernandes
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 08/57859-5 - Biorrational control of insect pests
Grantee:Maria Fátima das Graças Fernandes da Silva
Support Opportunities: Research Projects - Thematic Grants