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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)


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Allan Radaic ; Gustavo Oliveira Pugliese ; Guilherme Capiraço Campese ; Francisco Benedito Teixeira Pessine ; Marcelo Bispo de Jesus
Total Authors: 5
Document type: Journal article
Source: Química Nova; v. 39, n. 10, p. 1236-1244, Dez. 2016.

Although in recent years there has been an increasing amount of literature on nanotechnology and their clinical applications, it is still scarce a deep understanding of the interactions at the molecular levels between nanoparticles and cells. Studies demonstrating the underlying mechanisms of nanoparticles endocytosis, intracellular trafficking, and cellular processing are imperative to understand better how cells interact with those materials and their possible undesired effects, e.g. nanotoxicity. The rising awareness concerning nanoparticles applications and its interactions with the cellular environment is part of the new research field called Nanotoxicology. The cumulative knowledge in nanotoxicology will allow us to foresee toxic effects, establish regulations and limits for nanoparticles applications. In this work, we discuss the theoretical concepts about studying endocytosis and intracellular trafficking of nanoparticles. The nanoparticles-cell interactions are a multi-step process, which can be divided into nanoparticles' internalization, intracellular processing and triggering effects of nanomaterials on eukaryotic cells. Finally, we discuss the main techniques used to study this process: flow cytometry, use of endocytosis inhibitors and confocal microscopy. (AU)

FAPESP's process: 13/05432-6 - Molecular bases of endocytosis and cell death induced by biosynthetic silver nanoparticles in breast cancer cells
Grantee:Gustavo Oliveira Pugliese
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/03002-7 - Internalization and intracellular trafficking of nanoparticles: biological activity and nanotoxicological profile
Grantee:Marcelo Bispo de Jesus
Support type: Research Grants - Young Investigators Grants