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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro Evaluation of Copaifera oblongifolia Oleoresin Against Bacteria Causing Oral Infections and Assessment of Its Cytotoxic Potential

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Moraes, Thais da S. ; Leandro, Luis F. ; Silva, Larissa de O. ; Santiago, Mariana B. ; Souza, Ariana B. ; Furtado, Ricardo A. ; Tavares, Denise C. ; Veneziani, Rodrigo C. S. ; Ambrosio, Sergio R. ; Bastos, Jairo K. ; Martins, Carlos H. G.
Total Authors: 11
Document type: Journal article
Source: CURRENT PHARMACEUTICAL BIOTECHNOLOGY; v. 17, n. 10, p. 894-904, 2016.
Web of Science Citations: 7
Abstract

The oral cavity, which harbors more than 750 bacterial species, is one of the most diverse sites of the human body. Some of these bacteria have been associated with oral diseases, such as dental caries and endodontic infections. We report on the antimicrobial and cytotoxic activities of Copaifera oblongifolia oleoresin against bacteria that cause caries and endodontic infections. The aim of this study is to determine the minimum (MIC) and the bactericidal (MBC) inhibitory concentrations as well as the biofilm inhibition ability (through determination of MBIC50) of the C. oblongifolia oleoresin. This study also investigated the bactericidal kinetics (time-kill curves) and the synergistic effect of the C. oblongifolia oleoresin. Additionally, this study evaluated the cytotoxic activity of the oleoresin toward V79 cells by means of the colony-forming assay. The C. oblongifolia oleoresin gave promising MIC and MBC values, which ranged from 25 to 200 mu g/mL. Analysis of the MBIC50 values of the oleoresin revealed it displayed biofilm inhibitory activity against all the assayed bacteria. Analysis of the bactericidal kinetics showed different behaviors of the oleoresin against the tested bacteria at the different time intervals and concentrations assayed in this study. An additive effect of the oleoresin with chlorhexidine dihydrochloride occurred only for S. mitis and A. actinomycetemcomitans. The C. oblongifolia oleoresin showed cytotoxic activity at concentrations >= 625 mu g/mL. (AU)

FAPESP's process: 12/25237-0 - Kaurane-type diterpenes: activity against anaerobic bacteria and proteomics
Grantee:Carlos Henrique Gomes Martins
Support type: Regular Research Grants
FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
Grantee:Jairo Kenupp Bastos
Support type: Research Projects - Thematic Grants