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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Participation of MicroRNA-34a and RANKL on bone repair induced by poly(vinylidene-trifluoroethylene)/barium titanate membrane

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Author(s):
Lopes, Helena B. ; Ferraz, Emanuela P. ; Almeida, Adriana L. G. ; Florio, Pedro ; Gimenes, Rossano ; Rosa, Adalberto L. ; Beloti, Marcio M.
Total Authors: 7
Document type: Journal article
Source: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION; v. 27, n. 13, p. 1369-1379, 2016.
Web of Science Citations: 4
Abstract

The poly(vinylidene-trifluoroethylene)/barium titanate (PVDF) membrane enhances in vitro osteoblast differentiation and in vivo bone repair. Here, we hypothesized that this higher bone repair could be also due to bone resorption inhibition mediated by a microRNA (miR)/RANKL circuit. To test our hypothesis, the large-scale miR expression of bone tissue grown on PVDF and polytetrafluoroethylene (PTFE) membranes was evaluated to identify potential RANKL-targeted miRs modulated by PVDF. The animal model used was rat calvarial defects implanted with either PVDF or PTFE. At 4 and 8weeks, the bone tissue grown on membranes was submitted to a large-scale analysis of miRs by microarray. The expression of miR-34a and some of its targets, including RANKL, were evaluated by real-time polimerase chain reaction and osteoclast activity was detected by tartrate-resistant acid phosphatase (TRAP) staining. Among more than 250 miRs, twelve, including miR-34a, were simultaneously higher expressed (2 fold) at 4 and 8weeks on PVDF. The higher expression of miR-34a was concomitant with a reduced expression of all its evaluated targets, including RANKL. Additionally, more TRAP-positive cells were observed in bone tissue grown on PTFE compared with PVDF in both time points. In conclusion, our results suggest that the higher bone formation induced by PVDF could be, at least in part, triggered by a miR-34a increase and RANKL decrease, which may inhibit osteoclast differentiation and activity, and bone resorption. (AU)

FAPESP's process: 11/13623-0 - Effect of the Poly(Vinylidene-Trifluoroethylene)/Barium Titanate Membrane on the Bone Formation in Rat Cavariae Defects
Grantee:Helena Bacha Lopes
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/01622-5 - Large-scale analysis of the microRNAs expression in RNA samples obtained from newly formed bone tissue in contact with PTFE and PVDF membranes
Grantee:Helena Bacha Lopes
Support type: Scholarships abroad - Research Internship - Master's degree
FAPESP's process: 11/10658-8 - Effect of the poly(vinylidene-trifluoroethylene)/barium titanate membrane on the bone formation in rat cavariae defects
Grantee:Márcio Mateus Beloti
Support type: Regular Research Grants