Anti-inflammatory and anti-biofilm properties of ent-nemorosone from Brazilian geopropolis

Author(s)

da Cunha, Marcos Guilherme ; Franchin, Marcelo ; de Paula-Eduardo, Laila Facin ; Freires, Irlan Almeida ; Beutler, John A. ; de Alencar, Severino Matias ; Ikegaki, Masaharu ; Machado Tabchoury, Cinthia Pereira ; Cunha, Thiago Mattar ; Rosalen, Pedro Luiz

• Document type: Journal article
• Source: Journal of Functional Foods; v. 26, p. 27-35, OCT 2016.
• Web of Science Citations: 2

Abstract

ent-Nemorosone is a benzophenone isolated from geopropolis from Melipona scutellaris whose biological properties remain unexplored. Herein, we evaluated the anti-inflammatory activity of ent-nemorosone by in vivo neutrophil migration and TNF-alpha quantification, as well as by in vitro TNF-alpha quantification, ERK 1/2 phosphorylation, NF-kB activation and nuclear translocation of p65 in stimulated macrophages. ent-Nemorosone was also tested for its antimicrobial effects against Streptococcus mutans biofilm adhesion, polysaccharide production, and viability ent-Nemorosone reduced the influx of neutrophils and TNF-alpha release into the peritoneal cavity of mice, as well as the in vitro TNF-alpha levels, ERK 1/2 phosphorylation, NF-kB activation and nuclear p65 translocation. Furthermore, this compound inhibited biofilm adhesion and reduced the amount of extra- and intracellular polysaccharides synthesized by S. mutans. Thus, ent-nemorosone showed promising anti-inflammatory activity by acting on TNF-alpha release in macrophages, as well as anti-biofilm properties by reducing S. mutans polysaccharide production. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/22002-2 - Chemical elucidation of biocompounds and molecular targets of geopropolis on cancer cell lines
• Grantee: Marcos Guilherme da Cunha
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 12/22378-2 - In vivo and in vitro study of activity of isolated compounds from Brazilian propolis on modulation of neutrophils migration in the inflammatory process
• Grantee: Pedro Luiz Rosalen
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/23635-6 - Isolation and pharmacological evaluation of bioactive molecules from hexane fraction of M. scutellaris geopropolis
• Grantee: Marcos Guilherme da Cunha
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/16501-3 - Isolation and identification of bioactive compounds from geopropolis (Melipona scutellaris) bioguided by antimicrobial activity in dental biofilms
• Grantee: Laila Facin de Paula Eduardo
• Support Opportunities: Scholarships in Brazil - Master