| Full text | |
| Author(s): |
Silva, Rangel L.
;
Castanheira, Fernanda V.
;
Figueiredo, Jozi G.
;
Bassi, Gabriel S.
;
Ferreira, Sergio H.
;
Cunha, Fernando Q.
;
Cunha, Thiago M.
;
Kanashiro, Alexandre
Total Authors: 8
|
| Document type: | Journal article |
| Source: | Inflammation; v. 39, n. 4, p. 1405-1413, AUG 2016. |
| Web of Science Citations: | 2 |
| Abstract | |
The aim of this study was to identify the effect of beta-adrenergic receptor activation on neutrophil migration in experimental peritonitis elucidating the neuroimmune components involved such as nicotinic receptors and the spleen. Mice pre-treated with mecamylamine (nicotinic antagonist) and propranolol (beta-adrenergic antagonist) or splenectomized animals were treated with isoproterenol (beta-adrenergic agonist) prior to intraperitoneal injection of carrageenan. After 4 h, the infiltrating neutrophils and the local cytokine/chemokine levels were evaluated in the peritoneal lavage. The effect of isoproterenol on neutrophil chemotaxis was investigated in a Boyden chamber. Isoproterenol inhibited neutrophil trafficking, reducing the cytokine/chemokine release and neutrophil chemotaxis. Surprisingly, the isoproterenol effect on neutrophil migration was totally reverted by splenectomy and mecamylamine pre-treatment. In contrast, the inhibitory effect of nicotine on neutrophil migration was abrogated only by splenectomy but not by propranolol pre-treatment. Collectively, our data show that beta-adrenergic receptor activation regulates the acute neutrophil recruitment via splenic nicotinic receptor. (AU) | |
| FAPESP's process: | 13/08216-2 - CRID - Center for Research in Inflammatory Diseases |
| Grantee: | Fernando de Queiroz Cunha |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 11/20343-4 - Antiinflammatory cholinergic pathway: the role of neuroimmunomodulation in the control of inflammatory response |
| Grantee: | Alexandre Kanashiro |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 11/19670-0 - Mechanisms involved in the pathophysiology of rheumatoid arthritis, pain and sepsis |
| Grantee: | Fernando de Queiroz Cunha |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/01466-3 - The role of cholinergic anti-inflammatory pathway in the neutrophil migration in model of experimental peritonitis |
| Grantee: | Matheus Christovam Mangetti |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 12/04237-2 - Antiinflammatory Cholinergic Pathway: The role of neuroimmunomodulation in the control of inflammatory response. |
| Grantee: | Alexandre Kanashiro |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |