Novel integrative strategies through the aldoketo reductase from the termite Copto...
| Full text | |
| Author(s): Show less - |
Tramontina, Robson
;
Franco Cairo, Joao Paulo L.
;
Liberato, Marcelo V.
;
Mandelli, Fernanda
;
Sousa, Amanda
;
Santos, Samantha
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Rabelo, Sarita Candida
;
Campos, Bruna
;
Ienczak, Jaciane
;
Ruller, Roberto
;
Damasio, Andre R. L.
;
Squina, Fabio Marcio
Total Authors: 12
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| Document type: | Journal article |
| Source: | BIOTECHNOLOGY FOR BIOFUELS; v. 10, JAN 3 2017. |
| Web of Science Citations: | 6 |
| Abstract | |
Background: In nature, termites can be considered as a model biological system for biofuel research based on their remarkable efficiency for lignocellulosic biomass conversion. Redox enzymes are of interest in second-generation ethanol production because they promote synergic enzymatic activity with classical hydrolases for lignocellulose saccharification and inactivate fermentation inhibitory compounds produced after lignocellulose pretreatment steps. Results: In the present study, the biochemical and structural characteristics of the Coptotermes gestroi aldo-keto reductase (CgAKR-1) were comprehensively investigated. CgAKR-1 displayed major structural differences compared with others AKRs, including the differences in the amino acid composition of the substrate-binding site, providing basis for classification as a founding member of a new AKR subfamily (family AKR1 I). Immunolocalization assays with anti-CgAKR-1 antibodies resulted in strong fluorescence in the salivary gland, proventriculus, and foregut. CgAKR-1 supplementation caused a 32% reduction in phenolic aldehydes, such as furfural, which act as fermentation inhibitors of hemicellulosic hydrolysates, and improved ethanol fermentation by the xylose-fermenting yeast Scheffersomyces stipitis by 45%. We observed synergistic enzymatic interactions between CgAKR-1 and commercial cellulosic cocktail for sugarcane bagasse saccharification, with a maximum synergism degree of 2.17 for sugar release. Our data indicated that additive enzymatic activity could be mediated by reactive oxygen species because CgAKR-1 could produce hydrogen peroxide. Conclusion: In summary, we identified the founding member of an AKRI subfamily with a potential role in the termite digestome. CgAKR-1 was found to be a multipurpose enzyme with potential biotechnological applications. The present work provided a basis for the development and application of integrative and multipurpose enzymes in the bioethanol production chain. (AU) | |
| FAPESP's process: | 13/06336-0 - Study of the molecular basis of Carbohydrate Binding Modules (CBMs) from metagenomic screenings |
| Grantee: | Bruna Medéia de Campos |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 12/20549-4 - Secretion of heterologous glycoproteins in Aspergillus: effect of glycosylation pattern in functional parameters of glycosyl hydrolases |
| Grantee: | André Ricardo de Lima Damasio |
| Support type: | Program for Research on Bioenergy (BIOEN) - Young Investigators Grants |
| FAPESP's process: | 14/20576-7 - Characterization of auxiliary (redox) enzymes from the termite Coptotermes gestroi that cooperate with glycolytic hydrolases |
| Grantee: | Robson Tramontina |
| Support type: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/04105-4 - Structural and functional characterization of new cellulases, focusing in the relation between catalytic domains and CBMs |
| Grantee: | Marcelo Vizoná Liberato |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome |
| Grantee: | Fábio Márcio Squina |
| Support type: | Program for Research on Bioenergy (BIOEN) - Young Investigators Grants |