| Full text | |
| Author(s): |
Batista, Andrea N. L.
;
dos Santos-Pinto, Jose Roberto A.
;
Batista, Jr., Joao M.
;
Souza-Moreira, Tatiana M.
;
Santoni, Mariana M.
;
Zanelli, Cleslei F.
;
Kato, Massuo J.
;
Lopez, Silvia N.
;
Palma, Mario S.
;
Furlan, Maysa
Total Authors: 10
|
| Document type: | Journal article |
| Source: | Journal of Natural Products; v. 80, n. 5, p. 1275-1286, MAY 2017. |
| Web of Science Citations: | 4 |
| Abstract | |
Peperomia obtusifolia, an ornamental plant from the Piperaceae family, accumulates a series of secondary metabolites with interesting biological properties. From a biosynthesis standpoint, this species produces several benzopyrans derived from orsellinic acid, which is a polyketide typically found in fungi. Additionally, the chiral benzopyrans were reported as racemic and/or as diastereomeric mixtures, which raises questions about the level of enzymatic control in the cyclization step for the formation of the 3,4-dihydro-2H-pyran moiety. Therefore, this article describes the use of shotgun proteomic and transcriptome studies as well as phytochemical profiling for the characterization of the main biosynthesis pathways active in P. obtusifolia. This combined approach resulted the identification of a series of proteins involved in its secondary metabolism, including tocopherol cyclase and prenyltransferases, The activity of these enzymes was supported by the phytochemical profiling performed in different organs of-P. obtusifolia. However, the polyketide synthases possibly involved in the production of orsellinic acid could not be identified, suggesting that orsellinic acid may be produced by endophytes intimately associated with the plant. (AU) | |
| FAPESP's process: | 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery |
| Grantee: | Glaucius Oliva |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 11/51684-1 - System biology as experimental strategy for discovery of novel natural products in the fauna of venomous arthropods from São Paulo State |
| Grantee: | Mario Sergio Palma |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 14/25222-9 - Vibrational chiroptical spectroscopy for stereochemical characterization of small molecules and macromolecules |
| Grantee: | João Marcos Batista Junior |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 11/01003-8 - Proteomic studies in Peperomia obtusifolia (Piperaceae) |
| Grantee: | Andrea Nastri de Luca Batista |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |