Genetic Variants Involved in One-Carbon Metabolism: Polymorphism Frequencies and Differences in Homocysteine Concentrations in the Folic Acid Fortification Era

Author(s)

Steluti, Josiane ; Carvalho, Aline M. ; Carioca, Antonio A. F. ; Miranda, Andreia ; Gattas, Gilka J. F. ; Fisberg, Regina M. ; Marchioni, Dirce M.

• Document type: Journal article
• Source: NUTRIENTS; v. 9, n. 6 JUN 2017.
• Web of Science Citations: 2

Abstract

Folate and other B vitamins are essential co-factors of one-carbon metabolism, and genetic variants, such as polymorphisms, can alter the metabolism. Furthermore, the adoption of food fortification with folic acid showed a decrease of homocysteine concentration. The aim of this study was to investigate the frequencies of the polymorphisms of enzymes and carrier proteins involved in one-carbon metabolism, and to evaluate homocysteine concentrations in the presence of these genetic variants in a population exposed to mandatory food fortification with folic acid. Using data from a population-based cross-sectional study in Sao Paulo, Brazil, the study population comprised 750 participants above 12 years of age of both genders. A linear regression model was used to evaluate the homocysteine concentrations according to genetic variants and folate level. The results showed that the minor allelic frequencies were 0.33 for MTHFR (rs1801133), 0.24 for MTHFR (rs1801131), 0.19 for MTR (rs1805087), 0.42 for MTRR (rs1801394), 0.46 for RFC1 (rs1051266), and 0.47 for DHFR (19-bp deletion). The genetic variants of MTHFR 677C> T, MTRR 66A> G and RFC-1 80G> A were different according to race. The homocysteine concentrations increased in the CT and TT compared to CC genotypes of polymorphism MTHFR 677C> T in all populations, and differences between the homocysteine concentrations according to the genotypes of MTHFR 677C> T were observed regardless of folate level. (AU)

• FAPESP's process: 12/05505-0 - Standardization of methodology and analysis of unmetabolized folic acid after mandatory fortification with folic acid: population-based study in São Paulo City
• Grantee: Josiane Steluti
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 11/19788-1 - Assessment of folate status after mandatory folic acid fortification and health consequences: population-based study in the City of São Paulo
• Grantee: Dirce Maria Lobo Marchioni
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/19899-5 - Dietary intake and genetic variants of folate and its relation to folate, unmetabolized folic acid and homocysteine blood concentrations after mandoty folic acid fortification: population based study - ISA-Capital.
• Grantee: Josiane Steluti
• Support Opportunities: Scholarships in Brazil - Doctorate