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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Porcine pancreatic lipase hydrophobically adsorbed on octyl-silica: A robust biocatalyst for syntheses of xylose fatty acid esters

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Author(s):
Vescovi, Vinicius ; Carmello dos Santos, Jessica Bravin ; Tardioli, Paulo Waldir
Total Authors: 3
Document type: Journal article
Source: BIOCATALYSIS AND BIOTRANSFORMATION; v. 35, n. 4, p. 298-305, 2017.
Web of Science Citations: 2
Abstract

This work describes the immobilization of porcine pancreatic lipase (PPL), obtained from crude extract, on silica coated with octyl groups (OS) by interfacial adsorption, a simple and low-cost immobilization protocol. The biocatalyst PPL-OS was employed to the enzymatic preparation of fatty acid esters of d-xylose, a product used especially in the field of cosmetics and pharmacy, especially dermatology, improving the functionality of epidermal cells. The yields of the immobilization in terms of enzymatic activity and protein concentration (98% and 75%, respectively) suggested that PPL present in the crude extract was selectively immobilized on the octyl-silica support, which allowed the hyperactivation of the biocatalyst (recovered activity, 144%), a phenomenon that may be attributed to the interfacial activation of the enzyme on hydrophobic surfaces. The biocatalyst PPL-OS showed to be very robust in organic medium and at high temperature, which is an extremely important characteristic to produce sugar fatty acid esters from the industrial point of view. The syntheses of xylose fatty esters (oleate, caprylate and butyrate) yielded conversions around 70% after short reaction period (2h) at 60 degrees C in tert-butyl alcohol. The biocatalyst, even after incubation at 60 degrees C for 24h, could be reused in four esterification cycles of 2-h reaction at 60 degrees C, maintaining 100% of its catalytic activity. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 11/23194-0 - Enzymatic synthesis of fatty esters using heating assisted by microwave
Grantee:Paulo Waldir Tardioli
Support Opportunities: Regular Research Grants