Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in Sao Paulo state, Brazil

Author(s)

Antunes Murata, Fernando Henrique ; Ferreira, Marina Neves ; Pereira-Chioccola, Vera Lucia ; Junqueira Franco Spegiorin, Ligia Cosentino ; Meira-Strejevitch, Cristina da Silva ; Gava, Ricardo ; Silveira-Carvalho, Aparecida Perpetuo ; de Mattos, Luiz Carlos ; Brandao de Mattos, Cinara Cassia ; Grp, FAMERP Toxoplasma Res

• Document type: Journal article
• Source: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 89, n. 1, p. 13-19, SEP 2017.
• Web of Science Citations: 2

Abstract

Toxoplasmosis during pregnancy can have severe consequences. The use of sensitive and specific serological and molecular methods is extremely important for the correct diagnosis of the disease. We compared the ELISA and ELFA serological methods, conventional PCR (cPCR), Nested PCR and quantitative PCR (qPCR) in the diagnosis of Toxoplasma gondii infection in pregnant women without clinical suspicion of toxoplasmosis (GI = 94) and with clinical suspicion of toxoplasmosis (G2 = 53). The results were compared using the Kappa index, and the sensitivity, specificity, positive predictive value and negative predictive value were calculated. The results of the serological methods showed concordance between the ELISA and ELFA methods even though ELFA identified more positive cases than ELISA. Molecular methods were discrepant with cPCR using B22/23 primers having greater sensitivity and lower specificity compared to the other molecular methods. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 12/07716-9 - Serological and molecular investigation of Toxoplasma gondii in blood donors
• Grantee: Luiz Carlos de Mattos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/05302-8 - Isolation and genetic characterization of Toxoplasma gondii in Myrmecophaga tridactyla (Linnaeus, 1758)
• Grantee: Lilian Castiglioni
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/15879-8 - Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS)
• Grantee: Fernando Henrique Antunes Murata
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/07750-2 - Serological and molecular investigation of Toxoplasma gondii in blood donors
• Grantee: Fabiana Nakashima
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/09496-1 - New diagnostic strategies employed in symptomatic toxoplasmosis
• Grantee: Vera Lúcia Pereira Chioccola
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/01706-7 - Genotypic characterization of Toxoplasma gondii in samples of pregnant women in the northwest region of the state of São Paulo
• Grantee: Marina Neves Ferreira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation