Association between Diastolic Dysfunction with Inflammation and Oxidative Stress in Females ob/ob Mice

Author(s)

Sartori, Michelle ; Conti, Filipe F. ; Dias, Danielle da Silva ; dos Santos, Fernando ; Machi, Jacqueline F. ; Palomino, Zaira ; Casarini, Dulce E. ; Rodrigues, Bruno ; de Angelis, Kytia ; Irigoyen, Maria-Claudia

• Document type: Journal article
• Source: FRONTIERS IN PHYSIOLOGY; v. 8, AUG 23 2017.
• Web of Science Citations: 5

Abstract

Objective: To evaluate autonomic and cardiovascular function, as well as inflammatory and oxidative stress markers in ob/ob female mice. Methods: Metabolic parameters, cardiac function, arterial pressure (AP), autonomic, hormonal, inflammatory, and oxidative stressmarkers were evaluated in 12-weeks female wild-type (WT group) and ob/ob mice (OB group). Results: OB animals showed increased body weight, blood glucose, and triglyceride levels, along with glucose intolerance, when compared to WT animals. Ejection fraction (EF) and AP were similar between groups; however, the OB group presented diastolic dysfunction, as well as an impairment on myocardial performance index. Moreover, the OB group exhibited important autonomic dysfunction and baroreflex sensitivity impairment, when compared to WT group. OB group showed increased Angiotensin II levels in heart and renal tissues; decreased adiponectin and increased inflammatory markers in adipose tissue and spleen. Additionally, OB mice presented a higher damage to proteins and lipoperoxidation and lower activity of antioxidant enzymes in kidney and heart. Correlations were found between autonomic dysfunction with angiotensin II and inflammatory mediators, as well as between inflammation and oxidative stress. Conclusions: Our results showed that female adult ob/ob mice presented discrete diastolic dysfunction accompanied by autonomic disorder, which is associated with inflammation and oxidative stress in these animals. (AU)

• FAPESP's process: 10/17188-4 - Dysfunction associated mechanisms to a menopause and syndrome metabolic experimental model: role of aerobic and resistance exercise training
• Grantee: Janaina de Oliveira Brito
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/11223-6 - Time course of neuroimmune changes induced by chronic fructose consumption in normotensive and hypertensive rats: role of aerobic exercise training
• Grantee: Kátia de Angelis Lobo D Avila
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/15828-9 - Effects of combined exercise training in an experimental model of cardiometabolic disorders associated with ovarian hormone deprivation.
• Grantee: Filipe Fernandes Conti
• Support Opportunities: Scholarships in Brazil - Master