Soft nanocomposites of gelatin and poly(3-hydroxybutyrate) nanoparticles for dual drug release

Author(s)

Bini, Rafael A. ; Silva, Monica F. ; Varanda, Laudemir C. ; da Silva, Marcelo A. ; Dreiss, Cecile A.

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 157, p. 191-198, SEP 1 2017.
• Web of Science Citations: 9

Abstract

We developed a nanocomposite gel composed of gelatin and poly(3-hydroxybutyrate) polyineric nanoparticles (PNP) to be used as an injectable gel for the contemporaneous, dual sustained release of bioactive molecules. The hydrogel matrix was formed by a very simple process, using either the physical gelation of gelatin or the natural enzyme transglutaminase to covalently cross-link the gelatin chains in the presence of embedded PNP. Oscillatory rheological measurements showed that the addition of the PNP induced an increase in the storage modulus compared to pure gelatin gels, for both physical and chemical gels. Micrographs from scanning electron microscopy revealed that the presence of PNP disrupted the native structure of the gelatin chains in the hydrogel matrix. Dual drug encapsulation was achieved with curcumin (CM) in the PNP and naproxen sodium(NS) in the gelatin matrix. In vitro release studies showed that the hydrogel matrix acts both as a physical and chemical barrier, delaying the diffusion of the drugs. An initial burst release was observed in the first hours of the measurement, and around 90% was released on the third day for naproxen sodium. In free PNP, 82% of curcumin was relased after four days, while when PNP were embedded in the gelatin matrix only 40% was released over the same time period. Overall, these simple, sustainable soft nanocomposites show potential as an injectable co-sustained drug release system. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/01284-2 - Theranostic nanoparticles based in superparamagnetic iron oxide for skin cancer therapy by magnetic hyperthermia
• Grantee: Laudemir Carlos Varanda
• Support Opportunities: Regular Research Grants
• FAPESP's process: 07/07919-9 - Colloidal magnetic nanocrystals: synthesis of self-assembled and macromolecules-functionalised nanospheres, nanowires, and nanorods for advanced magnetic recording, biotechnological, and biomedical applications
• Grantee: Laudemir Carlos Varanda
• Support Opportunities: Research Grants - Young Investigators Grants