Gelatin-polycaprolactone nanocomposite for dual nitrix oxide release

• Grant number: 19/22827-0
• Support type: Scholarships abroad - Research Internship - Scientific Initiation
• Effective date (Start): January 07, 2020
• Effective date (End): March 23, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Marcelo Ganzarolli de Oliveira
• Grantee: Yasmim Raquel Lopes
• Supervisor abroad: Cecile Ayako Dreiss
• Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Local de pesquisa : King's College London, England
• Associated to the scholarship: 18/12552-1 - Spin coating technique applied to the coating of ventricular assist device with nitric oxide releasing polyester films, BP.IC

Abstract

In this study, gelatin/polycaprolactone nanoparticles (Gelatin/PCL-NPs) nanocomposites, charged with the hydrophilic (S-nitrosoglutathione, GSNO) and hydrophobic (S-nitroso-N-acetylpenicilamime, SNAP) NO donors will be prepared with the aim of obtaining a formulation with dual NO release profile: a faster NO release provided by GSNO, which will be present in the gelatin phase, followed by a slow NO release profile provided by SNAP which will be present in the polyester NPs phase. This approach is expected to lead to formulations that may allow tailoring the NO release rate to different applications, ranging from those which demand high rates of NO release such as for obtaining bactericidal action, to those which demand slow NO release at low doses such as for promoting wound healing. Previous works from the group of Prof. Cecile Dreiss at the Institute of Pharmaceutical Science, King's College, London, UK have shown that gelatin/polyhydroxybutirate nanocomposites were capable of providing dual release of an antioxidant and a NSAID, providing a proof of concept for the combination of gelatin and polyester nanoparticles for this purpose. In the present project PCL-NPs will be used as the polyester phase for the incorporation of SNAP and gelatin will be used for the incorporation of the water-soluble NO donor GSNO. The nanocomposites will be characterized regarding their rheological properties subjected to different hydration conditions to simulate their behavior while absorbing biological fluid after application along with the morphology of the PCL-NPs prepared. The NO release profiles of these formulations will be further characterized by chemiluminescence in the laboratory of Prof. Marcelo G. de Oliveira, after the return of the student to Brazil.