Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genetic diversity and structure in a major Brazilian annatto (Bixa orellana) germplasm bank revealed by microsatellites and phytochemical compounds

Full text
Dequigiovani, Gabriel ; Ferreyra Ramos, Santiago Linorio ; Alves-Pereira, Alessandro ; Fabri, Eliane Gomes ; Nogueira Carvalho, Paulo Roberto ; da Silva, Marta Gomes ; Vilela Nogueira Abdo, Maria Teresa ; Mello Martins, Antonio Lucio ; Clement, Charles Roland ; Veasey, Elizabeth Ann
Total Authors: 10
Document type: Journal article
Source: Genetic Resources and Crop Evolution; v. 64, n. 7, p. 1775-1788, OCT 2017.
Web of Science Citations: 3

Annatto (Bixa orellana L.) is a tropical crop indigenous to the Americas, probably Amazonia. Annatto is commercially valuable in the food and cosmetics industries as a natural dye used instead of synthetic dyes. In addition, annatto contains other important substances for human health, such as geranylgeraniol, tocotrienols and other carotenoids. The aim of the present study was to evaluate the genetic diversity of 63 accessions from the annatto germplasm bank at the Agronomic Institute (IAC), So Paulo, Brazil, using four phytochemical compounds and 16 microsatellite markers. Significant variation was observed for the phytochemical compounds, ranging from 2 to 7.31 g (100 g dry matter-DM)(-1) for bixin, 2.14-7.11 g (100 g DM)(-1) for lipids, 0.25-1.05 g (100 g DM)(-1) for tocotrienols, and 0.49-2.61 g (100 g DM)(-1) for geranylgeraniol content. A total of 73 alleles was observed in the molecular characterization with 16 microsatellite loci. We found higher expected heterozygosity than observed heterozygosity for all loci, indicating strong deficits of heterozygotes. For both molecular and phytochemical compounds, cluster and PCoA analyses tended to separate the accessions from Rondnia, northern Brazil, with only a few exceptions, from the Southwestern accessions. The same two groups were found in the Bayesian analysis with molecular data. Rondnia accessions showed higher values for all the phytochemical compounds and higher levels of genetic diversity. Some accessions presented bixin levels well above the average and are promising materials to be used in genetic improvement programs. (AU)

FAPESP's process: 13/08884-5 - Genetic structure and mating system of annatto (Bixa orellana L.) populations from the Brazilian Amazon and Central Brazil using microsatellite markers
Grantee:Gabriel Dequigiovanni
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/08307-5 - Genetic structure and mating system of local varieties and wild populations of annatto (Bixa orellana L.) in Brazilian Amazonia and Central Brazil using microsatellite markers
Grantee:Elizabeth Ann Veasey
Support type: Regular Research Grants
FAPESP's process: 11/10357-8 - Evaluation of geranylgeraniol content in different varieties of annatto (Bixa orellanna, L.). Geranylgeraniol separation and extraction from annatto seeds
Grantee:Paulo Roberto Nogueira Carvalho
Support type: Regular Research Grants
FAPESP's process: 11/05243-3 - Development of microsatellite loci for genetic diversity characterization of annatto (Bixa orellana) accessions
Grantee:Gabriel Dequigiovanni
Support type: Scholarships in Brazil - Master