| Full text | |
| Author(s): |
Maia, M. M.
;
Meira-Strejevitch, C. S.
;
Pereira-Chioccola, V. L.
;
de Hippolito, D. D. C.
;
Silva, V. O.
;
Brandao de Mattos, C. C.
;
Frederico, F. B.
;
Siqueira, R. C.
;
de Mattos, L. C.
;
Grp, FAMERP IAL Toxoplasma Res
Total Authors: 10
|
| Document type: | Journal article |
| Source: | PARASITE IMMUNOLOGY; v. 39, n. 10 OCT 2017. |
| Web of Science Citations: | 5 |
| Abstract | |
This study evaluated levels for mRNA expression of 7 cytokines in ocular toxoplasmosis. Peripheral blood mononuclear cells (PBMC) of patients with ocular toxoplasmosis (OT Group, n=23) and chronic toxoplasmosis individuals (CHR Group, n=9) were isolated and stimulated in vitro with T. gondii antigen. Negative controls (NC) were constituted of 7 PBMC samples from individuals seronegative for toxoplasmosis. mRNA expression for cytokines was determined by qPCR. Results showed a significant increase in mRNA levels from antigen stimulated PBMCs derived from OT Group for expressing IL-6 (at P<.005 and P<.0005 for CHR and NC groups, respectively), IL-10 (at P<.0005 and P<.005 for CHR and NC groups, respectively) and TGF- (at P<.005) for NC group. mRNA levels for TNF- and IL-12 were also upregulated in patients with OT compared to CHR and NC individuals, although without statistical significance. Additionally, mRNA levels for IL-27 and IFN- in PBMC of patients with OT were upregulated in comparison with NC individuals. Differences between OT and NC groups were statistically significant at P<.05 and P<.0005, respectively. (AU) | |
| FAPESP's process: | 13/15879-8 - Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS) |
| Grantee: | Fernando Henrique Antunes Murata |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/25650-8 - Class II HLA genes (DRB1 and DQB1) as risck factors for ocular toxoplasmosis |
| Grantee: | Luiz Carlos de Mattos |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/10050-5 - Serological, molecular and images in patients with active lesions or acute relapse of ocular infection by Toxoplasma gondii: a study follow-up |
| Grantee: | Mariana Previato |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 14/09496-1 - New diagnostic strategies employed in symptomatic toxoplasmosis |
| Grantee: | Vera Lúcia Pereira Chioccola |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/04803-6 - Cytokine expression in the modulation of cutaneous leishmaniasis and its interaction with clinical, epidemiological and laboratory findings |
| Grantee: | Daise Damaris Carnietto de Hippólito |
| Support Opportunities: | Scholarships in Brazil - Master |