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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Targeted-Analysis of β-Carboline Alkaloids in Passionfruit (“Maracujá”) by SBSE(PDMS)-LC/Flu and UHPLC-MS

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Author(s):
Vítor F. Freire [1] ; Gabriela R. Silva [2] ; Janete H. Yariwake [3]
Total Authors: 3
Affiliation:
[1] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil
[2] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil
[3] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil
Total Affiliations: 3
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 29, n. 4, p. 775-781, 2018-04-00.
Abstract

Samples of pulp and seeds of sour (P. edulis) and sweet (P. alata) passionfruit were subjected to targeted analysis to identify β-carboline alkaloids, using combined UHPLC-MS (ultrahigh performance liquid chromatography-mass spectrometry) and SBSE(PDMS) (stir bar sorption extraction using polydimethylsiloxane as stationary phase) for sample preconcentration. All the samples contained harmane and harmine; harmaline was found only in P. alata seeds and in P. edulis pulp, while harmalol was found only in P. alata seeds. An analysis of sweet passionfruit by SBSE(PDMS) combined with LC/Flu (liquid chromatography/fluorescence detection) indicated 1.03 × 10-1 ± 3.05 × 10-3 µg harmane L-1 pulp and 7.44 × 10-5 ± 2.55 × 10-6 µg harmane g-1 seeds, but harmine was not detectable by the quantitative method. Our findings underscore the need for further studies about passionfruit food safety, in view of the potential toxicity of β-carboline alkaloids. (AU)

FAPESP's process: 09/54040-8 - Acquisition of a high-resolution Orbitrap mass spectrometer for the discovery and structural analysis of biologically active compounds: applications in proteomics, biomarkers, synthesis, isolation, and characterization of natural products, studies of redox systems in food and enzymatic synthesis
Grantee:Emanuel Carrilho
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 13/21886-7 - Development of methods for identification and quantification of natural products in plant extracts, emphasizing SBSE techniques (SBSE/LC-Fluorescence and SBSE/UHPLC-MS) for the investigation of further passion fruit (maracujá) harmane alkaloids
Grantee:Janete Harumi Yariwake
Support Opportunities: Regular Research Grants