Pharmacological Properties of Vochysia Haenkeana (Vochysiaceae) Extract to Neutralize the Neuromuscular Blockade Induced by Bothropstoxin-I (Lys49 Phospholipase A(2)) Myotoxin

Author(s):Show less -

Harder, Carolina ^[1] ; de Oliveira, Akila Lara ^[2] ; Scriboni, Andreia Borges ^[3] ; Oliveira Cintra, Adelia Cristina ^[4] ; Schezaro-Ramos, Raphael ^[5] ; dos Santos, Marcio Galdino ^[6] ; Cogo-Mueller, Karina ^[7] ; Hashimoto Miura, Regina Yuri ^[1] ; Floriano, Rafael Stuani ^[5] ; Rostelato-Ferreira, Sandro ^[1] ; Oshima-Franco, Yoko ^[2]

Affiliation

^[1] Univ Paulista Unip, Inst Hlth Sci, Av Independencia 210, BR-18087100 Sorocaba, SP - Brazil

^[2] Univ Sorocaba Uniso, Rodovia Raposo Tavares Km 92-5, BR-18023000 Sorocaba, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Pharmacol, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[4] Sao Paulo Univ USP, Dept Clin & Toxicol Anal & Bromatol, Fac Pharmaceut Sci, Via Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Campinas, UNICAMP, Fac Med Sci, Dept Pharmacol, Rua Tessalia Vieira de Camargo 126, BR-13083887 Campinas, SP - Brazil

^[6] Tocantins Fed Univ UFT, Av NS 15, ALC NO14, 109 Norte, BR-77001090 Palmas, TO - Brazil

^[7] Univ Estadual Campinas, UNICAMP, Fac Pharmaceut Sci, Rua Candido Portinari 200, BR-13083871 Campinas, SP - Brazil

• Document type: Journal article
• Source: ADVANCED PHARMACEUTICAL BULLETIN; v. 7, n. 3, p. 433-439, SEP 2017.
• Web of Science Citations: 2

Abstract

Purpose: Bothrops snakes are responsible for more than 70 % of snakebites every year in Brazil and their venoms cause severe local and systemic damages. The pharmacological properties of medicinal plants have been widely investigated in order to discover new alternative treatments for different classes of diseases including neglected tropical diseases as envenomation by snakebites. In this work, we have investigated the ability of Vochysia haenkeana stem barks extract (VhE) to neutralize the neuromuscular effects caused by Bothropstoxin-I (BthTX-I), the major phospholipase A(2) (PLA(2)) myotoxin from B. jararacussu venom. Methods: The biological compounds of VhE were analysed under thin layer chromatography (TLC) and its neutralizing ability against BthTX-I was assessed through twitch-tension recordings and histological analysis in mouse phrenic nerve-diaphragm (PND) preparations. The antimicrobial activity of VhE was assessed against S. aureus, E. coli and P. aeruginosa strains. The aggregation activity of VhE was analysed under protein precipitation assay. Results: VhE showed the presence of phenolic compound visualized by blue trace under TLC. VhE abolished the neuromuscular blockade caused by BthTX-I applying the pre-toxin incubation treatment and partially neutralized the BthTX-I action under post-toxin incubation treatment; VhE contributed slightly to decrease the myotoxicity induced by BthTX-I. The neutralizing mechanism of VhE may be related to protein aggregation. VhE showed no antimicrobial activity. Conclusion: V. haenkeana extract which has no antimicrobial activity exhibited neutralizing ability against the neuromuscular blockade caused by BthTX-I and also contributed to decrease its myotoxicity. Protein aggregation involving phenolic compounds may be related in these protective effects. (AU)

• FAPESP's process: 15/01420-9 - Development and efficacy evaluation (in vitro and in vivo) of a hemoperfuser cartridge for the antiophidian treatment
• Grantee: Yoko Oshima Franco
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/08271-0 - IDENTIFICATION AND PHARMACODYNAMIC OF NEUROFACILITATING FRACTIONS OF Casearia sylvestris SW. AT THE NEUROMUSCULAR JUNCTION
• Grantee: Yoko Oshima Franco
• Support Opportunities: Regular Research Grants