| Full text | |
| Author(s): |
dos Santos, Ricardo Feliciano
[1]
;
Sposito, Marcel Bellato
[1]
;
Ayres, Matthew Ronald
[2]
;
Sosnowski, Mark Roman
[2, 3]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, Piracicaba, SP - Brazil
[2] South Australian Res & Dev Inst, Adelaide, SA - Australia
[3] Univ Adelaide, Waite Res Inst, Sch Agr Food & Wine, Adelaide, SA - Australia
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF PHYTOPATHOLOGY; v. 166, n. 3, p. 187-198, MAR 2018. |
| Web of Science Citations: | 6 |
| Abstract | |
Anthracnose caused by Elsinoe ampelina is one of the most important table grape diseases in humid regions in Brazil and Australia. The objective of this study was to characterize E.ampelina isolates from Brazil and Australia by means of phylogenetic analyses, morphological features and pathogenicity tests. Phylogenetic relationships among 35 isolates were determined based on a data set of internal transcribed spacer (ITS), histone H3 (HIS3) and elongation factor 1- (TEF) sequences. In phylogenetic tree analyses, using a combined ITS and TEF sequence alignment, all E.ampelina isolates were clustered together in a single well-supported clade. In contrast to the absence of genetic variability within ITS and TEF sequences, HIS3 sequences showed 54 polymorphic sites. The haplotype network generated from HIS3 data set showed four distinct haplotypes. EA1 was the predominant haplotype including 29 isolates from both countries. High genetic variability was observed in two Brazilian isolates, haplotype EA4, which may have lost the intron region during species evolution. Colony colours differed between Brazilian and Australian isolates, but showed similar wrinkled colony texture, absence of spores, sparse-to-absent white aerial mycelium and slow growth (0.049-0.060mm/day). Brazilian isolates produced conidia of 5.65x2.65m, larger than conidia from Australian isolates, which measured 5.14x2.30m. In pathogenicity tests, all nine Australian isolates inoculated were pathogenic on detached canes and potted vines of table grape. (AU) | |
| FAPESP's process: | 13/24003-9 - Grape diseases: epidemiology, damage assessment and control |
| Grantee: | Lilian Amorim |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/24472-1 - Etiology and epidemiology of anthracnose of grapevine |
| Grantee: | Ricardo Feliciano dos Santos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/01508-6 - Characterization of Elsinoe ampelina associated with anthracnose of grapevine in Brazil and Australia |
| Grantee: | Ricardo Feliciano dos Santos |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |