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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of Acetone Fraction From Buchenavia tomentosa Aqueous Extract and Gallic Acid on Candida albicans Biofilms and Virulence Factors

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Author(s):
Teodoro, Guilherme R. [1, 2] ; Gontijo, Aline V. L. [1, 2, 3] ; Salvador, Marcos J. [3] ; Tanaka, Marcia H. [1, 2] ; Brighenti, Fernanda L. [4] ; Delbem, Alberto C. B. [5] ; Delbem, Adina C. B. [5] ; Koga-Ito, Cristiane Y. [1, 2]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, Inst Sci & Technol Sao Jose dos Campos, Environm Engn Dept, Sao Paulo - Brazil
[2] Sao Paulo State Univ, Inst Sci & Technol Sao Jose dos Campos, Oral Biopathol Grad Program, Sao Paulo - Brazil
[3] Univ Estadual Campinas, Inst Biol, PPGBTPB, Dept Plant Biol, Campinas, SP - Brazil
[4] Sao Paulo State Univ, Araquara Fac Dent, Sao Paulo - Brazil
[5] Sao Paulo State Univ, Aracatuba Fac Dent, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: FRONTIERS IN MICROBIOLOGY; v. 9, APR 5 2018.
Web of Science Citations: 5
Abstract

A promising anti-Candida activity of Buchenavia tomentosa extracts was recently described. In the present work, experiments were carried out to determine the fraction with higher antifungal activity from a B. tomentosa extract. Acetone fraction (AF) was obtained from the aqueous extract from dried leaves (5 min/100 degrees C) and it was the most effective one. Gallic acid (GA) was identified by electrospray ionization mass spectrometry (ESI-MS) and also chosen to perform antifungal tests due to its promising activity on Candida albicans. Minimal inhibitory and fungicidal concentrations (MIC and MFC) were determined by broth microdilution technique. The effect on virulence factors of C. albicans was evaluated, and the cytotoxicity was determined. MIC50 and MIC90 values were both equal to 0.625 mg ml(-1) for AF and 2.5 and 5 mg ml(-1), respectively, for GA. AF and GA showed ability to inhibit C. albicans adherence and to disrupt 48 h-biofilm. AF and GA were effective in reducing the formation of hyphae of C. albicans SC5314. AF and GA decreased adherence of C. albicans to oral epithelial cells. AF and GA showed slight to moderate toxicity to Vero cells. This result suggests further studies for topic use of these compounds. AF, which contains a combination of several molecules, presented greater potential of antimicrobial activity than GA, with lower values of MIC and lower cytoxicity. (AU)

FAPESP's process: 12/16805-5 - ANTIFUNGAL ACTIVITY OF PHENOLYIC ENRICHED FRACTION OF Buchenavia tomentosa AND SOME ISOLATED SUBSTANCES BEFORE AND AFTER ENCAPSULATION WITH BETA-CYCLODEXTRIN
Grantee:Guilherme Rodrigues Teodoro
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/09666-4 - DEPLOYMENT OF ASSAYS FOR EVALUATION OF BIOPHARMACEUTIC CHARACTERISTICS AND BIOACTIVITY OF COMPOUNDS AND BIOACTIVE PRODUCTS USING IN VITRO MODELS OF INVASIVE CANDIDIASIS
Grantee:Aline Vidal Lacerda Gontijo
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/00037-1 - Antifungal activity of phenolic enriched fraction of Buchenavia tomentosa and some isolated substances before and after encapsulation in Beta-cyclodextrin
Grantee:CRISTIANE YUMI KOGA ITO
Support type: Regular Research Grants