Evaluation of photobiomodulation therapy associated with guided bone regeneration in critical size defects. In vivo study

Author(s)

Nicole Rosa de Freitas ^[1] ; Luísa Belluco Guerrini ^[2] ; Luis Augusto Esper ^[3] ; Michyele Cristhiane Sbrana ^[3] ; Gisele da Silva Dalben ^[4] ; Simone Soares ^[5] ; Ana Lúcia Pompéia Fraga de Almeida ^[7]

Affiliation

^[1] Univ Sao Paulo, Fac Odontol Bauru, Posgrad Reabilitacao Oral, Bauru, SP - Brazil

^[2] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Pós-Graduação em Reabilitação Oral - Brasil

^[3] Univ Sao Paulo, Hosp Reabilitacao Anomalies Craniofaciais, Secao Periodontia, Bauru, SP - Brazil

^[4] Univ Sao Paulo, Hosp Reabilitacao Anomalies Craniofaciais, Secao Odontopediat & Saude Colet, Bauru, SP - Brazil

^[5] Univ Sao Paulo, Fac Odontol Bauru, Dept Protese & Periodontia, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[6] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Prótese e Periodontia - Brasil

• Document type: Journal article
• Source: Journal of Applied Oral Science; v. 26, 2018-05-07.

Abstract

Abstract The repair of bone defects raises the interest of investigators in several health specialties. Grafting techniques with bone substitutes and laser therapies have been investigated to replace autogenous bone and accelerate the bone healing process. Objective To evaluate the effect of photobiomodulation therapy (PBMT) associated with guided bone regeneration (GBR) in critical size defects. Material and Methods The study was conducted on 80 male rats (Rattus norvegicus albinus, Wistar) submitted to surgical creation of a critical size defect on the calvaria, divided into eight study groups: group C (control - only blood clot); group M (collagen membrane); group PBMT (photobiomodulation therapy); group AB (autogenous bone); group AB+PBMT; group AB+M; group PBMT+M; group AB+PBMT+M. The animals were killed 30 days postoperatively. After tissue processing, bone regeneration was evaluated by histomorphometric analysis and statistical analyses were performed (Tukey test, p<0.05). Results All groups had greater area of newly formed bone compared to group C (9.96±4.49%). The group PBMT+M (achieved the greater quantity of new bone (64.09±7.62%), followed by groups PBMT (47.67±8.66%), M (47.43±15.73%), AB+PBMT (39.15±16.72%) and AB+PBMT+M (35.82±7.68%). After group C, the groups AB (25.10±16.59%) and AB+M (22.72±13.83%) had the smallest quantities of newly formed bone. The area of remaining particles did not have statistically significant difference between groups AB+M (14.93±8.92%) and AB+PBMT+M (14.76±6.58%). Conclusion The PBMT utilization may be effective for bone repair, when associated with bone regeneration techniques. (AU)

• FAPESP's process: 11/00779-2 - Analysis of healing of surgically created bone defects in rat calvaria treated with autogenous bone graft associated with low level laser. Histological and histometric study.
• Grantee: Isabella Luzia Medeiros
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 10/10538-0 - Analysis of healing of surgically created bone defects in rat calvaria treated with bovine grafts (Bio-Oss) associated with low level laser: histological and histometric study
• Grantee: Ana Lucia Pompeia Fraga de Almeida
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/13170-3 - Analysis of healing of surgically created bone defects in rat calvaria treated with bovine grafts (Bio-Oss) associated with low level laser. Histological and histometric study
• Grantee: Mércia Jussara da Silva Cunha
• Support Opportunities: Scholarships in Brazil - Master